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美国加利福尼亚中部和沿海地区污水固体中人类病毒核酸浓度。

Human viral nucleic acids concentrations in wastewater solids from Central and Coastal California USA.

机构信息

Department of Civil & Environmental Engineering, School of Engineering and Doerr School of Sustainability, Stanford University, Stanford, CA, USA.

Gangarosa Department of Environmental Health, Rollins School of Public Health, Emory University, Atlanta, GA, USA.

出版信息

Sci Data. 2023 Jun 22;10(1):396. doi: 10.1038/s41597-023-02297-7.

DOI:10.1038/s41597-023-02297-7
PMID:37349355
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10287720/
Abstract

We measured concentrations of SARS-CoV-2, influenza A and B virus, respiratory syncytial virus (RSV), mpox virus, human metapneumovirus, norovirus GII, and pepper mild mottle virus nucleic acids in wastewater solids at twelve wastewater treatment plants in Central California, USA. Measurements were made daily for up to two years, depending on the wastewater treatment plant. Measurements were made using digital droplet (reverse-transcription-) polymerase chain reaction (RT-PCR) following best practices for making environmental molecular biology measurements. These data can be used to better understand disease occurrence in communities contributing to the wastewater.

摘要

我们在美国加利福尼亚州中部的 12 个污水处理厂的污水固体中测量了 SARS-CoV-2、流感 A 和 B 病毒、呼吸道合胞病毒 (RSV)、猴痘病毒、人偏肺病毒、诺如病毒 GII 和辣椒轻斑驳病毒核酸的浓度。根据污水处理厂的不同,测量时间长达两年,每天进行一次测量。使用数字液滴(逆转录-)聚合酶链反应(RT-PCR)进行测量,遵循环境分子生物学测量的最佳实践。这些数据可用于更好地了解为污水做出贡献的社区中的疾病发生情况。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5d0/10287720/284debbd67a2/41597_2023_2297_Fig5_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5d0/10287720/284debbd67a2/41597_2023_2297_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5d0/10287720/6f1999d7696e/41597_2023_2297_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5d0/10287720/c8598912890f/41597_2023_2297_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5d0/10287720/7d11ac96564a/41597_2023_2297_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5d0/10287720/a378356df7f7/41597_2023_2297_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5d0/10287720/284debbd67a2/41597_2023_2297_Fig5_HTML.jpg

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