Rice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
Guangdong Key Laboratory of New Technology in Rice Breeding, Guangzhou, China.
BMC Plant Biol. 2023 Jun 22;23(1):332. doi: 10.1186/s12870-023-04347-z.
Bacterial leaf blight (BLB) is a highly destructive disease, causing significant yield losses in rice (Oryza sativa). Genetic variation is contemplated as the most effective measure for inducing resistance in plants. The mutant line T1247 derived from R3550 (BLB susceptible) was highly resistant to BLB. Therefore, by utilizing this valuable source, we employed bulk segregant analysis (BSA) and transcriptome profiling to identify the genetic basis of BLB resistance in T1247.
The differential subtraction method in BSA identified a quantitative trait locus (QTL) on chromosome 11 spanning a 27-27.45 Mb region with 33 genes and 4 differentially expressed genes (DEGs). Four DEGs (P < 0.01) with three putative candidate genes, OsR498G1120557200, OsR498G1120555700, and OsR498G1120563600,0.01 in the QTL region were identified with specific regulation as a response to BLB inoculation. Moreover, transcriptome profiling identified 37 resistance analogs genes displaying differential regulation.
Our study provides a substantial addition to the available information regarding QTLs associated with BLB, and further functional verification of identified candidate genes can broaden the scope of understanding the BLB resistance mechanism in rice.
细菌性条斑病（BLB）是一种极具破坏性的疾病，可导致水稻（Oryza sativa）严重减产。遗传变异被认为是诱导植物产生抗性的最有效措施。T1247 突变体系源自 R3550（BLB 敏感），对 BLB 高度抗性。因此，我们利用这一宝贵资源，采用 bulk segregant analysis（BSA）和转录组分析，鉴定 T1247 对 BLB 抗性的遗传基础。
BSA 的差异减法方法在第 11 号染色体上鉴定出一个包含 33 个基因和 4 个差异表达基因（DEGs）的 27-27.45 Mb 区域的数量性状位点（QTL）。在 QTL 区域内发现了 4 个差异表达基因（P < 0.01），其中 3 个具有潜在候选基因，OsR498G1120557200、OsR498G1120555700 和 OsR498G1120563600，0.01 对 BLB 接种具有特定的调控作用。此外，转录组分析鉴定出 37 个差异调节的抗性模拟基因。
本研究为 BLB 相关 QTL 的现有信息提供了重要补充，对鉴定出的候选基因进行进一步的功能验证可以拓宽对水稻 BLB 抗性机制的理解。
