Chung W L, Parish E J, Bone L W
J Parasitol. 1986 Apr;72(2):326-9.
Testosterone, progesterone and cholesterol were found in mixed sexes of the nematode Trichostrongylus colubriformis from goats, according to thin-layer, gas-liquid and high-performance liquid chromatography. The structure of these steroids was confirmed by proton nuclear magnetic resonance and mass spectroscopy. Melting points of the worms' steroids were similar to authentic standards of the steroids. Estradiol was not detected in worms from either goat sex. Cholesterol was about 0.08% of the worms' dry weight in helminths from either sex of host. Testosterone was 0.02% of the dry weight when worms were taken from male goats, but only 0.005% from female goats. Progesterone was not detected in worms from male goats, but was 0.005% of the dry weight of helminths from female hosts. Incubation of a worm preparation with tritiated steroids showed that progesterone was converted to 17-alpha-hydroxyprogesterone, based on retention during radioactive thin-layer and gas-liquid chromatography, and co-crystallization. Testosterone, cholesterol and 17-beta-estradiol were not metabolized.
根据薄层色谱法、气液色谱法和高效液相色谱法分析，在山羊体内的捻转血矛线虫（Trichostrongylus colubriformis）雌雄混合样本中发现了睾酮、孕酮和胆固醇。通过质子核磁共振和质谱分析确定了这些类固醇的结构。线虫类固醇的熔点与类固醇标准品相似。在山羊的雌雄样本线虫中均未检测到雌二醇。在来自任何性别的宿主的蠕虫中，胆固醇约占蠕虫干重的0.08%。当从雄性山羊体内获取线虫时，睾酮占干重的0.02%，但从雌性山羊体内获取的线虫中，睾酮仅占干重的0.005%。在雄性山羊的线虫中未检测到孕酮，但在雌性宿主的蠕虫中，孕酮占干重的0.005%。用氚标记的类固醇对线虫制剂进行孵育，结果显示，基于放射性薄层色谱法和气液色谱法中的保留情况以及共结晶现象，孕酮可转化为17-α-羟基孕酮。睾酮、胆固醇和17-β-雌二醇未发生代谢。
