CAS Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, China.
University of Chinese Academy of Sciences, Beijing, China.
Environ Microbiol. 2023 Nov;25(11):2592-2603. doi: 10.1111/1462-2920.16442. Epub 2023 Jun 22.
Soil arsenic (As) pollution not only decreases plant productivity but also soil quality, in turn hampering sustainable agricultural development. Despite the negative effects of As contamination on rice yield and quality being reported widely, the responses of microbial communities and co-occurrence networks in paddy soil to As pollution have not been explored. Here, based on high-throughput sequencing technologies, we investigated bacterial abundance and diversity in paddy soils with different levels of As contamination, and constructed associated microbial co-occurrence networks. As pollution reduced soil bacterial diversity significantly (p < 0.001). In addition, bioavailable As concentrations were negatively correlated with Actinobacteria and Acidobacteria relative abundance (p < 0.05). Conversely, As pollution had a positive relationship with Chloroflexi, Betaproteobacteria, and Bacteroidetes relative abundance (p < 0.05). Firmicutes relative abundance decreased with an increase in total As concentration. The ecological clusters and key groups in bacterial co-occurrence networks exhibited distinct trends with an increase in As pollution. Notably, Acidobacteria play an important role in maintaining microbial networks in As contaminated soils. Overall, we provide empirical evidence that As contamination influences soil microbial community structure, posing a threat to soil ecosystem health and sustainable agriculture.
土壤砷（As）污染不仅降低了植物的生产力，还降低了土壤质量，从而阻碍了可持续农业的发展。尽管已有广泛报道称砷污染对水稻产量和质量有负面影响，但土壤中微生物群落对砷污染的响应及其共生网络尚未得到探索。在这里，我们基于高通量测序技术，研究了不同砷污染水平稻田土壤中的细菌丰度和多样性，并构建了相关的微生物共生网络。砷污染显著降低了土壤细菌多样性（p<0.001）。此外，有效态 As 浓度与放线菌和酸杆菌相对丰度呈负相关（p<0.05）。相反，砷污染与绿弯菌门、β-变形菌纲和拟杆菌门相对丰度呈正相关（p<0.05）。厚壁菌门相对丰度随总 As 浓度的增加而减少。细菌共生网络中的生态聚类和关键组随砷污染程度的增加呈现出不同的趋势。值得注意的是，酸杆菌在维持砷污染土壤中的微生物网络方面发挥着重要作用。总的来说，我们提供了经验证据表明砷污染影响土壤微生物群落结构，对土壤生态系统健康和可持续农业构成威胁。
