Ahn Jin-Chul, Mo Sang Joon, Choi Min, Kim Bora, Cho Sung Bin
Medical Laser Research Center, College of Medicine, Dankook University, Cheonan, Korea.
R&D Center, Shenb Co., Ltd, Seoul, Korea.
Clin Cosmet Investig Dermatol. 2023 Jun 17;16:1559-1567. doi: 10.2147/CCID.S415679. eCollection 2023.
Various laser- and light-based devices have been introduced as complementary or alternative treatment modalities for dermatophytosis, particularly for finger or toenail onychomycosis.
This study aimed to comparatively evaluate the antifungal effects of 405-nm and 635-nm dual-band diode lasers using an in vivo guinea pig model of dermatophytosis.
A guinea pig model was developed by the repetitive application of fungal spore preparations to the back skin of guinea pigs. Dual-diode laser treatment was delivered to the guinea pig skin at a power of 24 mW at a wavelength of 405 nm and 18 mW at 635 nm for 12 min. The treatments were administered three times weekly for 2 weeks, and a mycological study was performed.
Mycological studies using scraped samples obtained from treatment groups A (N = 8) and B (N = 8) after dual-diode laser treatment revealed that seven of eight (87.5%) samples in each group had negative results for direct potassium hydroxide microscopy and fungal culture studies. Skin specimens from each infected laser-untreated guinea pig exhibited spongiotic psoriasiform epidermis with parakeratosis. Meanwhile, skin specimens from infected laser-treated guinea pigs in groups A and B demonstrated thinner epidermal thickness than those from infected untreated controls but thicker than those from uninfected treated controls without noticeable inflammatory cell infiltration in the dermis.
The guinea pig dermatophytosis model can be used to comparatively evaluate the efficacy and safety of various treatment modalities, including dual-diode lasers, for superficial fungal skin infection.
各种基于激光和光的设备已被引入作为皮肤癣菌病的辅助或替代治疗方式，特别是用于手指或脚趾甲的甲真菌病。
本研究旨在使用皮肤癣菌病的体内豚鼠模型，比较评估405纳米和635纳米双波段二极管激光的抗真菌效果。
通过将真菌孢子制剂反复应用于豚鼠背部皮肤建立豚鼠模型。双二极管激光治疗以405纳米波长24毫瓦、635纳米波长18毫瓦的功率照射豚鼠皮肤12分钟。每周进行三次治疗，持续2周，并进行真菌学研究。
对双二极管激光治疗后A组（N = 8）和B组（N = 8）治疗组刮取的样本进行真菌学研究发现，每组8个样本中有7个（87.5%）的直接氢氧化钾显微镜检查和真菌培养研究结果为阴性。每只未接受激光治疗的感染豚鼠的皮肤标本显示为海绵状银屑病样表皮伴角化不全。同时，A组和B组接受激光治疗的感染豚鼠的皮肤标本显示表皮厚度比未治疗的感染对照组薄，但比未感染的治疗对照组厚，真皮中无明显炎症细胞浸润。
豚鼠皮肤癣菌病模型可用于比较评估包括双二极管激光在内的各种治疗方式对浅表真菌皮肤感染的疗效和安全性。
