Department of Mycology, Pasteur Institute of Iran, Tehran 1316943551, Iran; Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Department of Nanobiotechnology, Pasteur Institute of Iran, Tehran 1316943551, Iran.
Diagn Microbiol Infect Dis. 2024 Dec;110(4):116555. doi: 10.1016/j.diagmicrobio.2024.116555. Epub 2024 Oct 9.
The emergence of high-resistance strains to known antifungal drugs has highlighted the urgency of developing novel therapies for chronic dermatophytosis as a global health problem. An experimental dermatophytosis model in guinea pigs was developed to investigate the in vivo wound healing effects of cold atmospheric plasma (CAP) on T. rubrum skin invasion. Guinea pigs were experimentally infected with T. rubrum and wound healing was evaluated at 1, 4, 8 and 12 days post infection in the CAP-treated, terbinafine-treated and non-treated controls. Our results showed that CAP strongly inhibited the fungal virulence in vitro in culture media and in vivo on the skin lesions of experimentally infected guinea pigs even more efficient than that of terbinafine, resulting in complete wound healing at 8 days post infection. These results indicate that CAP would be considered as a promising tool comparable to conventional chemical therapies, for the treatment of drug-resistant chronic dermatophytosis caused by T. rubrum.
高耐药菌株的出现突显了开发新型疗法治疗慢性皮肤癣菌病作为全球健康问题的紧迫性。本研究建立了豚鼠实验性皮肤癣菌病模型，以研究冷等离子体（CAP）对红色毛癣菌皮肤入侵的体内伤口愈合作用。豚鼠经实验性感染红色毛癣菌，在感染后 1、4、8 和 12 天分别评估 CAP 处理、特比萘芬处理和未处理对照组的伤口愈合情况。结果显示，CAP 在体外培养基中和体内实验性感染豚鼠的皮肤损伤中均强烈抑制真菌毒力，甚至比特比萘芬更有效，导致感染后 8 天完全愈合。这些结果表明，CAP 可作为一种有前途的工具，与传统化学疗法相当，用于治疗由红色毛癣菌引起的耐药性慢性皮肤癣菌病。
