Zhu Huiting, Zhang Yanli, Du Shiyu, Wang Huifen, Zheng Yue
Department of Internal Medicine, Hebei Medical University, Shijiazhuang, China.
Department of Gastroenterology, First Hospital of Qinhuangdao, Qinhuangdao, China.
Front Med (Lausanne). 2023 Jun 7;10:1183484. doi: 10.3389/fmed.2023.1183484. eCollection 2023.
To compare and analyze the mucosal metabolites and mucosal microbiota of different parts of colon in patients with IBS.
A total of 10 patients with IBS-D and six healthy controls (HC) were enrolled. All enrolled participants underwent two biopsies of the ileocecal and sigmoid colon during colonoscopy. Metabolomic profiling of one piece of tissue was conducted using desorption electrospray ionization-mass spectrometry (DESI-MS), and the gut flora of the other piece was examined using 16S rRNA sequencing. The metabolic profiles and flora of the ileocecal and sigmoid colonic mucosa in each group were further analyzed in this study.
(1) Principal components analysis (PCA) indicated that mucosal metabolites did not differ in different parts of the colon in either the IBS-D or HC groups. (2) In the mucosal microbiome analyses, no differences between the microbiota of the two parts of the colon were found by using Principal Co-ordinates Analysis (PCoA). In IBS group, comparing with sigmoid mucosa, the chao1 richness indice was higher and the Shannon index was lower in the ileocecal mucosa ( = 0.40, = 0.22). However, in the HC group, microbiome analysis of the ileocecal mucosa showed lower values for Chao 1 and Shannon indices than those of the sigmoid colon mucosa ( = 0.06, = 0.86). (3) Compared with the HC group, 1,113 metabolic signal peaks were upregulated, whereas 594 metabolites were downregulated in the IBS-D samples. Moreover, the PCA of the metabolites showed significant separation between the IBS-D and HC groups. (4) Chao1 expression was significantly higher in the mucosal microbiota with IBS-D than in the HC ( = 0.03). The Shannon index was lower in IBS-D, but the difference was not statistically significant ( = 0.53). PCoA revealed a significant difference in the microflora structure between the IBS-D and HC groups.
The mucosal metabolic profile and mucosal flora structure of the colon were similar, despite different locations in IBS and healthy subjects. IBS had abnormal colonic mucosal metabolism and flora disturbances.
比较和分析肠易激综合征（IBS）患者结肠不同部位的黏膜代谢物和黏膜微生物群。
共纳入10例腹泻型肠易激综合征（IBS-D）患者和6名健康对照者（HC）。所有纳入的参与者在结肠镜检查期间对回盲部和乙状结肠进行了两次活检。使用解吸电喷雾电离质谱（DESI-MS）对其中一块组织进行代谢组分析，另一块组织的肠道菌群则采用16S rRNA测序进行检测。本研究进一步分析了每组回盲部和乙状结肠黏膜的代谢谱和菌群情况。
（1）主成分分析（PCA）表明，IBS-D组和HC组结肠不同部位的黏膜代谢物无差异。（2）在黏膜微生物组分析中，通过主坐标分析（PCoA）未发现结肠两个部位的微生物群存在差异。在IBS组中，与乙状结肠黏膜相比，回盲部黏膜的chao1丰富度指数较高，而香农指数较低（P = 0.40，P = 0.22）。然而，在HC组中，回盲部黏膜的微生物组分析显示Chao 1和香农指数的值低于乙状结肠黏膜（P = 0.06，P = 0.86）。（3）与HC组相比，IBS-D样本中有1113个代谢信号峰上调，而594种代谢物下调。此外，代谢物的PCA显示IBS-D组和HC组之间有明显分离。（4）IBS-D患者黏膜微生物群中的Chao1表达明显高于HC组（P = 0.03）。IBS-D组的香农指数较低，但差异无统计学意义（P = 0.53）。PCoA显示IBS-D组和HC组之间的微生物群结构存在显著差异。
尽管IBS患者和健康受试者结肠的位置不同，但其结肠黏膜代谢谱和黏膜菌群结构相似。IBS存在结肠黏膜代谢异常和菌群紊乱。
