Stenlund Hans, Nilholm Clara, Chorell Elin, Roth Bodil, D'Amato Mauro, Ohlsson Bodil
Umeå Plant Science Centre (UPSC), Department of Plant Physiology, Umeå University, 90187 Umeå, Sweden.
Department of Clinical Sciences, Lund University, 22100 Lund, Sweden.
Metabolites. 2021 Jul 4;11(7):440. doi: 10.3390/metabo11070440.
A 4-week dietary intervention with a starch- and sucrose-restricted diet (SSRD) was conducted in patients with irritable bowel syndrome (IBS) to examine the metabolic profile in relation to nutrient intake and gastrointestinal symptoms. IBS patients were randomized to SSRD intervention ( = 69) or control continuing with their ordinary food habits ( = 22). Food intake was registered and the questionnaires IBS-symptoms severity scale (IBS-SSS) and visual analog scale for IBS (VAS-IBS) were completed. Metabolomics untargeted analysis was performed by gas chromatography mass spectrometry (GC-MS) and liquid chromatography mass spectrometry (LC-MS) in positive and negative ionization modes. SSRD led to marked changes in circulating metabolite concentrations at the group level, most prominent for reduced starch intake and increased polyunsaturated fat, with small changes in the control group. On an individual level, the correlations were weak. The marked reduction in gastrointestinal symptoms did not correlate with the metabolic changes. SSRD was observed by clear metabolic effects mainly related to linoleic acid metabolism, fatty acid biosynthesis, and beta-oxidation.
对肠易激综合征（IBS）患者进行了为期4周的淀粉和蔗糖限制饮食（SSRD）干预，以研究与营养摄入和胃肠道症状相关的代谢谱。IBS患者被随机分为SSRD干预组（n = 69）或继续保持普通饮食习惯的对照组（n = 22）。记录食物摄入量，并完成肠易激综合征症状严重程度量表（IBS-SSS）和肠易激综合征视觉模拟量表（VAS-IBS）问卷调查。采用气相色谱-质谱联用（GC-MS）和液相色谱-质谱联用（LC-MS）在正离子和负离子模式下进行代谢组学非靶向分析。在组水平上，SSRD导致循环代谢物浓度发生显著变化，最显著的是淀粉摄入量减少和多不饱和脂肪增加，而对照组变化较小。在个体水平上，相关性较弱。胃肠道症状的显著减轻与代谢变化无关。观察到SSRD具有明显的代谢效应，主要与亚油酸代谢、脂肪酸生物合成和β-氧化有关。
