采用钴镍钨酸盐对生物样本中的曲马多和血清素进行定量分析，以评估镇痛药对神经递质的影响。

Quantification of tramadol and serotonin by cobalt nickel tungstate in real biological samples to evaluate the effect of analgesic drugs on neurotransmitters.

机构信息

Department of Biochemistry, Bahauddin Zakariya University, Multan, 60800, Pakistan.

HEJ Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, 75270, Pakistan.

出版信息

Sci Rep. 2023 Jun 23;13(1):10239. doi: 10.1038/s41598-023-37053-9.

In this work, CoNiWO nanocomposite was used as an electrochemical sensor for the simultaneous electrochemical detection of tramadol and serotonin. The nanocomposite was synthesized using a hydrothermal method and characterized via XRD, SEM, TGA, Zeta, UV, and FTIR. The sensor was developed by depositing CoNiWO-NPs onto the glassy carbon electrode surface. Tramadol and serotonin were detected by employing cyclic voltammetry (CV), differential pulse voltammetry (DPV), electrochemical impedance spectroscopy (EIS), and chronoamperometry. Analytes were detected at different pH, concentrations, and scan rates. The prepared sensor showed a 0-60 µM linear range, with a LOD of 0.71 µM and 4.29 µM and LOQ of 14.3 µM and 2.3 µM for serotonin and tramadol, respectively. Finally, the modified electrode (CoNiWO-GCE) was applied to determine tramadol and serotonin in biological samples.

在这项工作中，CoNiWO 纳米复合材料被用作电化学传感器，用于同时电化学检测曲马多和血清素。该纳米复合材料是通过水热法合成的，并通过 XRD、SEM、TGA、Zeta、UV 和 FTIR 进行了表征。传感器是通过将 CoNiWO-NPs 沉积到玻碳电极表面而开发的。通过循环伏安法 (CV)、差分脉冲伏安法 (DPV)、电化学阻抗谱 (EIS) 和计时安培法检测曲马多和血清素。在不同的 pH 值、浓度和扫描速率下检测分析物。制备的传感器在 0-60 μM 线性范围内显示出线性关系，对于血清素和曲马多，LOD 分别为 0.71 μM 和 4.29 μM，LOQ 分别为 14.3 μM 和 2.3 μM。最后，将修饰电极（CoNiWO-GCE）用于测定生物样品中的曲马多和血清素。