大肠杆菌噬菌体P2晚期控制基因ogr。DNA序列及产物鉴定。
Coliphage P2 late control gene ogr. DNA sequence and product identification.
作者信息
Birkeland N K, Lindquist B H
出版信息
J Mol Biol. 1986 Apr 5;188(3):487-90. doi: 10.1016/0022-2836(86)90170-1.
PMID:3735430
Abstract
The bacteriophage P2 late control gene ogr was cloned and precisely localized by deletion analysis in vitro. The DNA sequence of the ogr gene containing the ogr1 mutation was determined. The sequence translates into a basic protein of a molecular weight of 8300. Plasmids overproducing the ogr gene product were constructed, and the ogr gene product was identified by polyacrylamide gel electrophoresis.
摘要
通过体外缺失分析克隆并精确定位了噬菌体P2晚期控制基因ogr。测定了含有ogr1突变的ogr基因的DNA序列。该序列翻译为分子量为8300的碱性蛋白质。构建了过量表达ogr基因产物的质粒，并通过聚丙烯酰胺凝胶电泳鉴定了ogr基因产物。
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