Li Tai, Wang Meihui, Cui Rufei, Li Bingchen, Wu Tong, Liu Yonglong, Geng Gui, Xu Yao, Wang Yuguang
Heilongjiang Sugar Beet Engineering Technology Research Center, College of Advanced Agriculture and Ecological Environment, Heilongjiang University, 74Xuefu Road, Harbin 150080, China; Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region, College of Life Sciences, Heilongjiang University, 74Xuefu Road, Harbin 150080, China.
College of Earth and Environmental Sciences, the University of Queensland, Brisbane, Queensland 4072, Australia.
Ecotoxicol Environ Saf. 2023 Jun 21;262:115172. doi: 10.1016/j.ecoenv.2023.115172.
Waterlogging has been shown to have a significant inhibitory effect on plant growth. However, the response mechanisms of the soil environment of sugar beet seedlings under waterlogging conditions still need to be fully understood. This study aimed to investigate the effects of waterlogging treatments on the content of effective nutrients and the microbial communities in the rhizosphere and non-rhizosphere using high-throughput sequencing. We set up waterlogging and non-waterlogging treatments, sampled sugar beet seedlings after 10 days of waterlogging, determined the effective soil nutrients in the rhizosphere and non-rhizosphere of the plants, and analyzed the differences in microbial diversity at ten days of waterlogging. The results showed that waterlogging significantly affected available potassium (AK) content. The Ak content of waterlogged soil was significantly higher than that of non-waterlogged soil. Waterlogging caused no significant difference in available nitrogen (AN) content and pH. Moreover, the plant growth-promoting bacteria Pseudomonas was significantly enriched in sugar beet waterlogged rhizospheres compared with the non-waterlogged ones. Similarly, the harmful fungi Gibellulopsis and Alternaria were enriched in sugar beet non-waterlogged rhizosphere. The network analysis revealed that waterlogging built a less complex root-microbial network than non-waterlogging. These findings implied that sugar beets subjected to waterlogging stress were enriched with beneficial microorganisms in the rhizosphere, potentially alleviating the stress.
涝渍已被证明对植物生长有显著抑制作用。然而，水涝条件下甜菜幼苗土壤环境的响应机制仍需充分了解。本研究旨在通过高通量测序研究涝渍处理对根际和非根际有效养分含量及微生物群落的影响。我们设置了涝渍和非涝渍处理，在涝渍10天后采集甜菜幼苗样本，测定植株根际和非根际土壤有效养分，并分析涝渍10天时微生物多样性的差异。结果表明，涝渍显著影响速效钾（AK）含量。涝渍土壤的AK含量显著高于非涝渍土壤。涝渍对速效氮（AN）含量和pH值无显著影响。此外，与非涝渍甜菜根际相比，促植物生长细菌假单胞菌在涝渍甜菜根际显著富集。同样，有害真菌拟粘帚霉属和链格孢属在非涝渍甜菜根际富集。网络分析表明，涝渍构建的根-微生物网络比非涝渍的更简单。这些发现表明，遭受涝渍胁迫的甜菜根际有益微生物富集，可能缓解胁迫。
