State Key Laboratory of Plant Diversity and Prominent Crops, Guangdong Provincial Key Laboratory of Applied Botany, Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China; South China National Botanical Garden, Guangzhou 510650, China; University of Chinese Academy of Sciences, Beijing 100049, China.
State Key Laboratory of Plant Diversity and Prominent Crops, Guangdong Provincial Key Laboratory of Applied Botany, Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China; South China National Botanical Garden, Guangzhou 510650, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Food Chem. 2023 Nov 15;426:136618. doi: 10.1016/j.foodchem.2023.136618. Epub 2023 Jun 17.
Pectin can improve the bioaccessibility of icaritin as a nanocarrier, and ultrasound can modify the pectin structure. However, the interaction between ultrasound-modified pectin (UMP) and icaritin remains unclearly. In this work, the effects of UMP on the physiochemical properties of icaritin/pectin micelles (IPMs) were investigated. The IPMs prepared with UMP (UMP-IPMs) showed lower encapsulation efficiencies and loading capacities, comparing with native IPMs. UMP-IPMs had smaller particle sizes (325-399 nm) than native IPMs (551 nm). The Mw, viscosity, G' and G" of pectin were determined. NMR spectra indicated that the repeating unit in pectins remained consistently before and after ultrasound treatment, and 7-OH of icaritin was involved in hydrogen bond formation with pectin. The larger chemical shift movement of 6-H and 7-OH for U3-IPMs than P0-IPMs suggested that stronger hydrogen bond interaction between icaritin and pectin. UMP-IPMs exhibited stronger anti-proliferation activities against HepG2 cells than native IPMs.
果胶可以作为纳米载体提高淫羊藿次苷的生物利用度，超声可以修饰果胶结构。然而，超声改性果胶（UMP）与淫羊藿次苷之间的相互作用尚不清楚。在这项工作中，研究了 UMP 对淫羊藿次苷/果胶胶束（IPMs）理化性质的影响。与天然 IPMs 相比，用 UMP 制备的 IPMs（UMP-IPMs）的包封效率和载药量较低。与天然 IPMs（551nm）相比，UMP-IPMs 的粒径较小（325-399nm）。测定了果胶的 Mw、粘度、G'和 G"。NMR 谱表明，超声处理前后果胶的重复单元保持一致，并且 7-OH 参与了与果胶的氢键形成。与 P0-IPMs 相比，U3-IPMs 的 6-H 和 7-OH 的化学位移移动更大，表明淫羊藿次苷与果胶之间的氢键相互作用更强。与天然 IPMs 相比，UMP-IPMs 对 HepG2 细胞的增殖抑制活性更强。
