Jiangsu Engineering Laboratory for Breeding of Special Aquatic Organisms, Huai'yin Normal University, Huai'an 223300, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China; Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China.
Jiangsu Engineering Laboratory for Breeding of Special Aquatic Organisms, Huai'yin Normal University, Huai'an 223300, China.
Comp Biochem Physiol Part D Genomics Proteomics. 2023 Sep;47:101105. doi: 10.1016/j.cbd.2023.101105. Epub 2023 Jun 19.
As an important aquaculture fish in the Heilongjiang River Basin, Pseudobagrus ussuriensis has high economic value, and all-male culture is beneficial to the economic development of this fish. In this study, the transcriptomes of gonads in males and females were analyzed, and some genes related to gonad development were found. A total of 82,931 unigenes were found (average length 1504 bp, N50 1829 bp). In addition, 4689 differentially expressed genes (DEGs; including 1424 genes upregulated and 3265 genes downregulated in males) were identified. Some genes associated with testis development (such as Dmrt1 and Ropn1l) were significantly upregulated in males, while genes related to ovary development (such as Wnt2, PLC, Cyp19a, ZP3) were significantly downregulated in males, demonstrating that these genes have a crucial influence on gonad development in P. ussuriensis. Some signaling pathways related to gonad development were found, such as the Wnt pathway and oocyte meiosis. The results of RNA-seq obtained in this study provide theoretical data for elucidating the potential mechanism of gonad development of P. ussuriensis and reliable genomic data for the establishment of mono-sex breeding of P. ussuriensis.
作为黑龙江流域重要的水产养殖鱼类，乌苏里拟鲿具有很高的经济价值，全雄养殖有利于该鱼类的经济发展。本研究对雌雄个体的性腺转录组进行了分析，找到了一些与性腺发育相关的基因。共发现了 82931 条 unigenes（平均长度 1504bp，N50 为 1829bp）。此外，还鉴定出了 4689 个差异表达基因（DEGs；包括在雄性中上调表达的 1424 个基因和下调表达的 3265 个基因）。一些与睾丸发育相关的基因（如 Dmrt1 和 Ropn1l）在雄性中显著上调，而与卵巢发育相关的基因（如 Wnt2、PLC、Cyp19a、ZP3）在雄性中显著下调，表明这些基因对乌苏里拟鲿的性腺发育有重要影响。还发现了一些与性腺发育相关的信号通路，如 Wnt 通路和卵母细胞减数分裂。本研究的 RNA-seq 结果为阐明乌苏里拟鲿性腺发育的潜在机制提供了理论数据，为建立乌苏里拟鲿的单性养殖提供了可靠的基因组数据。
