School of Life Sciences, Jiangsu Engineering Laboratory for Breeding of Special Aquatic Organisms, Huaiyin Normal University, Huaian, 223300, China.
Fish Physiol Biochem. 2021 Apr;47(2):565-581. doi: 10.1007/s10695-021-00932-x. Epub 2021 Feb 1.
The Ussuri catfish (Pseudobagrus ussuriensis) has an XX/XY sex determination system but its sex determination gene(s) remain unknown. To better understand the molecular sex determination mechanism, transcriptome analysis was conducted to obtain sex-related gene expression profiles. Transcriptome analyses were made of male and female developing/differentiating gonads by high-throughput RNA sequencing, including gonads from fish given an estradiol-induced sex reversal treatment. A total of 81,569 unigenes were assembled and 39,904 were significantly matched to known unique proteins by comparison with public databases. Twenty specifically expressed and 142 differentially expressed sex-related genes were extracted from annotated data by comparing the treatment groups. These genes are involved in spermatogenesis (e.g., Dnali1, nectin3, klhl10, mybl1, Katnal1, Eno4, Mns1, Spag6, Tsga10, Septin7), oogenesis (e.g., Lagr5, Fmn2, Npm2, zar1, Fbxo5, Fbxo43, Prdx4, Nrip1, Lfng, Atrip), gonadal development/differentiation (e.g., Cxcr4b, Hmgb2, Cftr, Ch25h, brip1, Prdm9, Tdrd1, Star, dmrt1, Tut4, Hsd17b12a, gdf9, dnd, arf1, Spata22), and estradiol response (e.g., Mmp14, Lhcgr, vtg1, vtg2, esr2b, Piwil1, Aifm1, Hsf1, gdf9). Dmrt1 and gdf9 may play an essential role in sex determination in P. ussuriensis. The expression patterns of six random genes were validated by quantitative real-time PCR, which confirmed the reliability and accuracy of the RNA-seq results. These data provide a valuable resource for future studies of gene expression and for understanding the molecular mechanism of sex determination/differentiation and gonadal development/differentiation (including hormone-induced sexual reversal) in Ussuri catfish. This has the potential to assist in producing monosex Ussuri catfish to increase aquacultural productivity.
乌苏里江拟鲿（Pseudobagrus ussuriensis）具有 XX/XY 性别决定系统，但性别决定基因仍不清楚。为了更好地理解分子性别决定机制，进行了转录组分析以获得性别相关的基因表达谱。通过高通量 RNA 测序对雄性和雌性发育/分化性腺进行转录组分析，包括接受雌二醇诱导性性别反转处理的鱼类性腺。总共组装了 81569 个基因，通过与公共数据库比较，有 39904 个显著匹配已知的独特蛋白质。通过比较处理组，从注释数据中提取了 20 个特异性表达和 142 个差异表达的性别相关基因。这些基因参与精子发生（例如，Dnali1、nectin3、klhl10、mybl1、Katnal1、Eno4、Mns1、Spag6、Tsga10、Septin7）、卵子发生（例如，Lagr5、Fmn2、Npm2、zar1、Fbxo5、Fbxo43、Prdx4、Nrip1、Lfng、Atrip）、性腺发育/分化（例如，Cxcr4b、Hmgb2、Cftr、Ch25h、brip1、Prdm9、Tdrd1、Star、dmrt1、Tut4、Hsd17b12a、gdf9、dnd、arf1、Spata22）和雌二醇反应（例如，Mmp14、Lhcgr、vtg1、vtg2、esr2b、Piwil1、Aifm1、Hsf1、gdf9）。Dmrt1 和 gdf9 可能在 P. ussuriensis 的性别决定中发挥重要作用。通过定量实时 PCR 验证了六个随机基因的表达模式，这证实了 RNA-seq 结果的可靠性和准确性。这些数据为未来的基因表达研究以及理解乌苏里江拟鲿的性别决定/分化和性腺发育/分化（包括激素诱导的性反转）的分子机制提供了有价值的资源。这有可能帮助生产单性乌苏里江拟鲿，以提高水产养殖产量。
