Muñoz-Almagro Nerea, Molina-Tijeras Jose Alberto, Montilla Antonia, Vezza Teresa, Sánchez-Milla María, Rico-Rodríguez Fabián, Villamiel Mar
Instituto de Investigación en Ciencias de la Alimentación (CIAL) (CSIC-UAM) CEI (CSIC+UAM), Nicolás Cabrera, 9, Campus de la Universidad Autónoma de Madrid, 28049 Madrid, Spain.
Departamento de Farmacología, Centro de Investigación Biomédica (CIBM), Facultad de Farmacia, Universidad de Granada, 18071 Granada, Spain.
Int J Biol Macromol. 2023 Aug 15;246:125505. doi: 10.1016/j.ijbiomac.2023.125505. Epub 2023 Jun 22.
Inflammatory bowel disease (IBD) is a public health challenge and the use of pectin for symptom amelioration is a promising option. In this work, sunflower pectin has been extracted without (CHP) and with assistance of ultrasound (USP) using sodium citrate as a food-grade extracting agent. At optimal conditions (64 °C, 23 min) the highest yield was obtained with ultrasound application (15.5 vs. 8.1 %). Both pectins were structurally characterized by H NMR, HPSEC-ELSD, FT-IR and GC-FID. Unlike CHP, USP showed a lower molecular weight, higher galacturonic acid, lower degree of methyl-esterification and, overall, higher viscosity. These characteristics could affect the anti-inflammatory activity of pectins, evaluated using DSS-induced IBD model mice. So, USP promoted the defence (ICAM-1) and repair of the gastrointestinal mucosa (TFF3, ZO-1) more effectively than CHP. These results demonstrate the potential amelioration of acute colitis in IBD mice through USP supplementation. Taking into account the biomarkers analysed, these results demonstrate, for the first time, the positive impact of sunflower pectin extracted by ultrasound under very soft conditions on inflammatory bowel disease that might open up new possibilities in the treatment of this serious pathology.
炎症性肠病（IBD）是一项公共卫生挑战，使用果胶改善症状是一个有前景的选择。在本研究中，以柠檬酸钠作为食品级提取剂，分别在无超声（CHP）和超声辅助（USP）的条件下提取了向日葵果胶。在最佳条件（64°C，23分钟）下，超声处理获得了最高产率（15.5%对8.1%）。通过1H NMR、HPSEC-ELSD、FT-IR和GC-FID对两种果胶进行了结构表征。与CHP不同，USP显示出较低的分子量、较高的半乳糖醛酸、较低的甲酯化程度以及总体上较高的粘度。这些特性可能会影响果胶的抗炎活性，通过葡聚糖硫酸钠（DSS）诱导的IBD模型小鼠进行评估。因此，USP比CHP更有效地促进了胃肠道黏膜的防御（ICAM-1）和修复（TFF3、ZO-1）。这些结果表明，通过补充USP可改善IBD小鼠的急性结肠炎。考虑到所分析的生物标志物，这些结果首次证明了在非常温和的条件下通过超声提取的向日葵果胶对炎症性肠病具有积极影响，这可能为治疗这种严重疾病开辟新的可能性。
