Instituto de Investigación en Ciencias de la Alimentación (CIAL) (CSIC-UAM) CEI (CSIC+UAM). Campus de la Universidad Autónoma de Madrid, Nicolás Cabrera 9 28049-Madrid, Spain.
Instituto de Cerámica y Vidrio (ICV), Consejo Superior de Investigaciones Científicas (CSIC), 28049-Madrid, Spain.
Molecules. 2019 Jan 22;24(3):392. doi: 10.3390/molecules24030392.
In this work, the efficient extraction of pectin from sugar beet by-products (pressed, ensiled and dried pulp), by using an acid method or a commercial cellulose, is accomplished. The extraction method had an impact on the pectin monomeric composition, mainly in xylose, arabinose, and galacturonic acid content, as determined by GC-FID. FTIR and SEC analyses allowed the determination of similar degrees of methoxylation and molecular weights, respectively, in the extracted pectins. The acid extraction of pectin in the ensiled by-product led to the highest yield (19%) with a galacturonic acid content of 46%, whereas the application of the enzymatic extraction method resulted in a lower yield (13%) but higher galacturonic acid content (72%). Moreover, the stability in aqueous solution as well as the emulsifying activity index was higher for pectin extracted by the acid method, whereas the viscosity was higher in pectin extracted by the enzymatic method. To the best of our knowledge, this is the first study analyzing the physicochemical properties and exploring the potential reuse of ensiled and dried by-products from sugar beet industry for the extraction of pectin to be further used in the food and pharmaceutical areas.
本工作采用酸法或商业纤维素从糖甜菜副产物(压榨、青贮和干燥的果肉)中高效提取果胶。提取方法对果胶的单一组分有影响,主要是通过 GC-FID 测定木糖、阿拉伯糖和半乳糖醛酸的含量。FTIR 和 SEC 分析分别确定了提取果胶的相似程度的甲氧基化和分子量。在青贮副产物中用酸提取果胶的产率最高(19%),半乳糖醛酸含量为 46%,而酶提取方法的应用导致产率较低(13%),但半乳糖醛酸含量较高(72%)。此外,酸法提取的果胶在水溶液中的稳定性和乳化活性指数较高,而酶法提取的果胶的粘度较高。据我们所知,这是首次分析青贮和干燥糖甜菜工业副产物的理化性质,并探索其用于提取果胶的潜在再利用,以进一步用于食品和制药领域。