Institute of Special Animal and Plant Sciences, Chinese Academy of Agriculture Sciences, Changchun, 130112, China.
Jilin Province Key Laboratory on Chemistry and Biology of Natural Drugs in Changbai Mountain, School of Life Sciences, Northeast Normal University, Changchun, 130024, China.
Sci Rep. 2020 Feb 27;10(1):3595. doi: 10.1038/s41598-020-60339-1.
A natural low-methoxyl pectin (LAHP), was extracted with oxalic acid solution from dried heads of sunflower (Helianthus annuus L.). The single-factor experiments and response surface methodology (RSM) were used to optimize LAHP extraction conditions. The extraction yield of LAHP was 18.83 ± 0.21%, and the uronic acid content was 85.43 ± 2.9% obtained under the optimized conditions (temperature of 96 °C, time of 1.64 h, oxalic acid concentration of 0.21%). Experimentally obtained values were in agreement with those predicted by RSM model, indicating suitability of the employed model and the success of RSM in optimizing the extraction conditions. LAHP has been characterized by ash content, degree of esterification (DE), galacturonic acid (GalA) content, molecular weight and intrinsic viscosity meanwhile commercial low-methoxyl pectin (CLMP) as comparison. This study finds out a potential source of natural LMP which expands the application scope of sunflower heads. It is an efficient reuse of waste resources and provides a novel thought to explore the natural resources for food and pharmaceutical applications.
从干燥的向日葵（Helianthus annuus L.）头部用草酸溶液提取天然低甲氧基果胶（LAHP）。采用单因素实验和响应面法（RSM）优化 LAHP 提取条件。在优化条件（温度为 96°C，时间为 1.64h，草酸浓度为 0.21%）下，LAHP 的提取率为 18.83±0.21%，糖醛酸含量为 85.43±2.9%。实验值与 RSM 模型预测值吻合，表明所采用的模型适宜，RSM 成功优化了提取条件。同时对灰分含量、酯化度（DE）、半乳糖醛酸（GalA）含量、分子量和特性粘度等特性进行了分析，与商品低甲氧基果胶（CLMP）进行了比较。本研究发现了一种天然 LMP 的潜在来源，扩大了向日葵头的应用范围。这是对废物资源的有效再利用，为探索天然资源在食品和医药应用方面提供了新的思路。
