Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, PR China.
Clinical Laboratory, Hebei General Hospital, Shijiazhuang, 050000, PR China.
Anal Chim Acta. 2023 Sep 1;1272:341436. doi: 10.1016/j.aca.2023.341436. Epub 2023 Jun 8.
Photocathodic biosensor possesses excellent anti-interference capability in bioanalysis, which however suffers from high electron-hole recombination rate with low photocurrent. Herein, a high-performance inorganic organic PHT@C@ZnO nanosphere with cascade energy band arrangement was synthesized as photoactive signal probe, which inherited the advantages of inorganic strong optical absorptivity and organic high mobility for photo-generated holes. Specifically, the well-matched band gap endowed not only the improved life for light generated carrier and promoted separation of electron-hole pairs, but also the expansion of charge-depletion layer, significantly improving the photoelectric conversion efficiency for acquiring an extremely high photocathodic signal that increased by 30 times compared with individual materials. Accordingly, by integrating with the efficient amplification of DNA nanonet derived from clamped hybrid chain reaction (C-HCR), a sensitive PHT@C@ZnO nanosphere based photocathodic biosensor was proposed for accurate detection of p53. The experimental results showed that the biosensor had a wide detection range from 0.1 fM to 10 nM and a low detection limit of 0.37 fM toward p53, offering a new avenue to construct sensitive PEC platform with superior anti-interference ability and hold a prospective application in early disease diagnosis and biological analysis.
基于光生载流子寿命延长和电子空穴对分离效率提高的光电化学生物传感器用于超灵敏检测 p53
光电阴极生物传感器在生物分析中具有出色的抗干扰能力，但存在光电流低和电子空穴复合率高的问题。在此，我们合成了一种具有级联能带排列的高性能无机-有机 PHT@C@ZnO 纳米球作为光活性信号探针，它继承了无机材料强吸光性和有机材料光生空穴迁移率高的优点。具体来说，匹配的能带间隙不仅提高了光生载流子的寿命，促进了电子空穴对的分离，而且扩展了电荷耗尽层，显著提高了光电转换效率，从而获得了极高的光电阴极信号，比单个材料提高了 30 倍。因此，通过整合源于夹式杂交链式反应 (C-HCR) 的高效 DNA 纳米网放大，我们提出了一种基于 PHT@C@ZnO 纳米球的灵敏光电阴极生物传感器，用于超灵敏检测 p53。实验结果表明，该生物传感器对 p53 的检测范围很宽，从 0.1 fM 到 10 nM，检测限低至 0.37 fM，为构建具有优异抗干扰能力的灵敏 PEC 平台提供了新途径，并有望在早期疾病诊断和生物分析中得到应用。
