College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China; Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan, 430070, China.
College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
Anal Chim Acta. 2023 Sep 1;1272:341501. doi: 10.1016/j.aca.2023.341501. Epub 2023 Jun 12.
In this study, a new technique was developed for visual and precise identification of Salmonella using phage T156-mediated aggregation of gold nanoparticles. The phage binds to gold nanoparticles in a dispersed and stable state under high NaCl concentrations. When Salmonella is introduced, the phage specifically recognizes and adsorbs the targeted bacteria, causing the AuNPs to undergo a discoloration reaction resulting in aggregation, which enables Salmonella visualization. The method has a detection range of 3.8 × 10-3.8 × 10 CFU/mL and a limit of detection of 38 CFU/mL and can produce results in approximately 80 min. The technique was also tested on field samples, including spiked lettuce, and was found to be accurate with a recovery rate of 81.0-119.2% and relative standard deviations ranging from 3.3% to 14.7%. Notably, this technique utilizes phages as recognition elements in colorimetric methods, offering simplicity, speed, and the ability to effectively distinguish between live and dead Salmonella. It demonstrates remarkable sensitivity, specificity, and accuracy. Furthermore, it presents a novel avenue for the rapid detection of other pathogenic bacteria.
在本研究中，开发了一种新的技术，使用噬菌体 T156 介导的金纳米颗粒聚集来可视化和精确识别沙门氏菌。在高 NaCl 浓度下，噬菌体以分散和稳定的状态结合到金纳米颗粒上。当引入沙门氏菌时，噬菌体特异性地识别并吸附目标细菌，导致 AuNPs 发生变色反应从而聚集，从而使沙门氏菌可视化。该方法的检测范围为 3.8×10-3.8×10 CFU/mL，检测限为 38 CFU/mL，大约 80 分钟即可得出结果。该技术还在现场样本（包括污染生菜）上进行了测试，结果准确，回收率为 81.0-119.2%，相对标准偏差为 3.3%-14.7%。值得注意的是，该技术在比色法中利用噬菌体作为识别元件，具有简单、快速的特点，并能够有效区分活和死的沙门氏菌。它表现出显著的灵敏度、特异性和准确性。此外，它为快速检测其他病原菌提供了新途径。
