Department of Oral and Maxillofacial Surgery, Aalborg University Hospital and Department of Clinical Medicine, The Faculty of Medicine, Aalborg University, Aalborg, Denmark.
Department of Dentistry and Oral Health, Section of Oral Radiology, Faculty of Health, Aarhus University, Aarhus, Denmark.
J Craniomaxillofac Surg. 2023 May;51(5):321-331. doi: 10.1016/j.jcms.2023.05.011. Epub 2023 Jun 12.
The objective was to test the hypothesis of no difference in radiographic outcome after maxillary sinus floor augmentation (MSFA) with allogeneic adipose tissue-derived stem cells (ASCs) seeded on deproteinized bovine bone mineral (DBBM) (test) compared with excipient on DBBM (control). Eighteen minipigs were assigned into three groups of six animals and euthanised after one month (T1), two months (T2), and four months (T3), respectively. Each maxillary sinus was randomly allocated to either test or control with an equal volume of graft. Computed tomography scans (CTs) after MSFA (T0) were compared with CTs after euthanasia to evaluate graft volume (GV) changes and bone density (BD) using three-dimensional measurements and Hounsfield units. GV was larger in test compared with control at T1 (P = 0.046), whereas GV was larger in control compared with test at T3 (P = 0.01). BD increased from T0 to T1-T3 (P < 0.001) with both treatments. Higher BD was observed in control compared with test at T3 (P = 0.01), while no significant difference was observed at T1 and T2. Conclusively, the present study demonstrate that allogeneic ASCs seeded on DBBM in conjunction with MSFA seemed not to improve the radiographic outcome compared with excipient on DBBM. However, radiological outcomes need to be supplemented by bone histomorphometry before definitive conclusions can be provided about the beneficial use of allogeneic ASCs seeded on DBBM in conjunction with MSFA compared with DBBM alone.
目的是检验在脱蛋白牛骨矿物质（DBBM）上接种同种异体脂肪组织源性干细胞（ASCs）与在 DBBM 上接种赋形剂（对照）对上颌窦底提升（MSFA）后影像学结果无差异的假设。将 18 只小型猪随机分为三组，每组 6 只，分别于 1 个月（T1）、2 个月（T2）和 4 个月（T3）安乐死。每个上颌窦均随机分配到试验组或对照组，每组等量移植物。在 MSFA 后（T0）进行计算机断层扫描（CT），并与安乐死后的 CT 进行比较，使用三维测量和亨氏单位评估移植物体积（GV）变化和骨密度（BD）。在 T1 时，试验组的 GV 大于对照组（P=0.046），而在 T3 时，对照组的 GV 大于试验组（P=0.01）。两种治疗方法的 BD 均从 T0 增加到 T1-T3（P<0.001）。在 T3 时，对照组的 BD 高于试验组（P=0.01），而在 T1 和 T2 时则无显著差异。总之，本研究表明，与 DBBM 上的赋形剂相比，在 DBBM 上接种同种异体 ASCs 联合 MSFA 似乎并不能改善影像学结果。然而，在提供关于与单独使用 DBBM 相比，在 DBBM 上接种同种异体 ASCs 联合 MSFA 对骨修复有益的最终结论之前，还需要通过骨组织形态计量学来补充影像学结果。
