Personalised Oncology Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia.
Department of Medical Biology, The University of Melbourne, Parkville, VIC, Australia.
Methods Mol Biol. 2023;2691:185-198. doi: 10.1007/978-1-0716-3331-1_14.
Glioma can be modelled in the murine brain through the induction of genetically engineered mouse models or intracranial transplantation. Gliomas (oligodendroglioma and astrocytoma) are thought to arise from neuronal and glial progenitor populations in the brain and are poorly infiltrated by immune cells. An improved understanding of oligodendrocytes, astrocytes, and the immune environment throughout tumor development will enhance the analysis and development of brain cancer models. Here, we describe the isolation and analysis of murine brain cell types using a combination of flow cytometry and quantitative RT-PCR strategies to analyze these individual cell populations in vivo.
脑胶质瘤可以通过诱导基因工程小鼠模型或颅内移植在鼠脑中建模。人们认为脑胶质瘤（少突胶质细胞瘤和星形细胞瘤）起源于脑内神经元和神经胶质前体细胞，并被免疫细胞浸润不良。更好地了解少突胶质细胞、星形胶质细胞和肿瘤发展过程中的免疫环境将增强脑癌模型的分析和开发。在这里，我们描述了使用流式细胞术和定量 RT-PCR 策略相结合来分离和分析鼠脑细胞类型，以分析这些细胞群体在体内的情况。
