Yin Dong-Mei, Yuan Dai, Sun Rui-Jie, Xu Hong-Zhi, Hun Shou-Yong, Sui Xiao-Hui, Shan Ning-Ning
Department of Blood Transfusion, Shandong Provincial Hospital, Shandong University, Jinan, Shandong, 250021, China.
Department of Blood Transfusion, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, 250021, China.
Clin Proteomics. 2023 Jun 24;20(1):24. doi: 10.1186/s12014-023-09413-0.
Immune thrombocytopenia (ITP) is a common autoimmune disease characterized by loss of immune tolerance to platelet autoantigens leading to excessive destruction and insufficient production of platelets.
Quantitative liquid chromatography tandem mass spectrometry (LC-MS/MS) was performed to detect the differentially expressed proteins in bone marrow samples from active ITP patients and normal controls.
Our bioinformatic analysis identified two upregulated proteins (ORM1 and vWF) and two downregulated proteins (PPBP and SPARC) related to immune function. The four proteins were all found to be related to the tumor necrosis factor (TNF) -α signalling pathway and involved in the pathogenesis of ITP in KEGG pathway analysis.
Bioinformatics analysis identified differentially expressed proteins in bone marrow that are involved in the TNF-α signalling pathway and are related to the activation of immune function in ITP patients. These findings could provide new ideas for research on the loss of immune tolerance in ITP patients.
免疫性血小板减少症（ITP）是一种常见的自身免疫性疾病，其特征是对血小板自身抗原失去免疫耐受，导致血小板过度破坏和生成不足。
采用定量液相色谱串联质谱法（LC-MS/MS）检测活动性ITP患者和正常对照者骨髓样本中差异表达的蛋白质。
我们的生物信息学分析确定了两种与免疫功能相关的上调蛋白（ORM1和vWF）和两种下调蛋白（PPBP和SPARC）。在KEGG通路分析中发现这四种蛋白均与肿瘤坏死因子（TNF）-α信号通路相关，并参与ITP的发病机制。
生物信息学分析确定了骨髓中差异表达的蛋白质，这些蛋白质参与TNF-α信号通路，与ITP患者免疫功能激活相关。这些发现可为研究ITP患者免疫耐受丧失提供新思路。
