Nacey J N, Horsfall D J, Delahunt B, Marshall V R
J Urol. 1986 Sep;136(3):706-9. doi: 10.1016/s0022-5347(17)45032-4.
A cell culture technique for assessing the toxicity of urinary catheters is described. The inhibitory effect of catheter extracts on the uptake of 3H-labelled thymidine by L929 mouse fibroblasts and human prostate derived epithelial cells in culture was measured. The concentration of extract which depressed uptake to 50 per cent of control (IC50) was determined for catheters of different composition. When compared to the degree of inflammation caused by the same catheters in an animal model, a significant correlation was observed (rs = -0.87). As a result of this correlation, the cell culture technique has the potential to be used as a valid alternative to the animal model.
本文描述了一种用于评估导尿管毒性的细胞培养技术。测量了导尿管提取物对培养的L929小鼠成纤维细胞和人前列腺来源上皮细胞摄取3H标记胸苷的抑制作用。针对不同成分的导尿管，测定了使摄取量降低至对照的50%（IC50）时的提取物浓度。当与相同导尿管在动物模型中引起的炎症程度进行比较时，观察到显著相关性（rs = -0.87）。基于这种相关性，该细胞培养技术有潜力作为动物模型的有效替代方法。
