Higher Institution of Centre of Excellence (HICoE), Tropical Infectious Diseases Research and Education Centre (TIDREC), Universiti Malaya, 50603, Kuala Lumpur, Malaysia.
International Center for Island Studies, Kagoshima University, Korimoto 1-21-24, Kagoshima, 890-8580 Japan.
Trop Biomed. 2023 Mar 1;40(1):88-100. doi: 10.47665/tb.40.1.007.
Simulium (Gomphostilbia) okinawense Takaoka and S. (G.) tokarense Takaoka, both from the Nansei Islands, Japan, were morphologically reexamined and genetically analysed by using the COI gene sequences. The female, male, pupa and mature larva of the two species are redescribed. Morphological reexamination shows that both species are more similar to species in the S. asakoae species-group than to those in the S. ceylonicum species-group, by having a medium-long female sensory vesicle, yellow tuft hairs (S. (G.) okinawense) or yellow tuft hairs mixed with a few to several dark hairs (S. (G.) tokarense) at the base of the radial vein in the female and male, and medium-long larval postgenal cleft. However, the body of the male ventral plate (viewed ventrally) is parallel-sided (S. (G.) okinawense) or parallelsided or slightly narrowed (S. (G.) tokarense) and not emarginated basally, differing from those of most species in the S. asakoae species-group. Our genetic analysis shows that S. (G.) tokarense is in the S. asakoae species-group, and S. (G.) okinawense formed a separate sister clade with other members of the S. asakoae species-group with high bootstrap support. From the results of morphological and genetic analysis combined, S. (G.) okinawense and S. (G.) tokarense are transferred from the S. ceylonicum species-group to the S. asakoae species-group.
日本琉球群岛的拟蚋属冲绳亚种 Simulium (Gomphostilbia) okinawense Takaoka 和托卡雷亚种 S. (G.) tokarense Takaoka，通过 COI 基因序列进行了形态学再检查和遗传分析。重新描述了这两个物种的雌性、雄性、蛹和成熟幼虫。形态学再检查表明，这两个物种与 S. asakoae 种组的物种更为相似，而不是与 S. ceylonicum 种组的物种更为相似，因为它们的雌性感觉囊较长，雄性和雌性的径向静脉基部有黄色簇毛（S. (G.) okinawense）或黄色簇毛与几根至几根深色毛发混合（S. (G.) tokarense），以及中等长的幼虫后颏裂。然而，雄性腹面腹板（腹面观察）的体侧是平行的（S. (G.) okinawense）或平行或略窄（S. (G.) tokarense），而不是基部有缺刻，与 S. asakoae 种组的大多数物种不同。我们的遗传分析表明，S. (G.) tokarense 属于 S. asakoae 种组，而 S. (G.) okinawense 与 S. asakoae 种组的其他成员形成了一个单独的姐妹分支，具有高的自举支持。从形态学和遗传分析的结果结合来看，S. (G.) okinawense 和 S. (G.) tokarense 从 S. ceylonicum 种组转移到 S. asakoae 种组。
