Faculty of Agricultural, Livestock and Environmental Sciences, Autonomous University of Guerrero, Iguala, Guerrero, Mexico, CP 40040.
National Center for Disciplinary Research in Animal Health and Safety. (CENID-SAI-INIFAP), Jiutepec, Morelos, Mexico, CP 62574.
Trop Biomed. 2023 Mar 1;40(1):108-114. doi: 10.47665/tb.40.1.017.
Haemonchus contortus (Hc) is a hematophagous parasite affecting the health and productivity of flocks. The administration of chemical anthelmintic drugs (AH) is the common method of deworming; however, generates resistance in the parasites to AH and it is a public health risk due to drug residues in milk, meat and sub-products. Natural compounds from plants are explored to diminish this parasitosis, improving their health and productivity, without the negative effects of AH. Ipomoea genus is a group of climbing plants belonging to the Convulvulaceae family possessing perennial leaves and tuberous roots. Medicinal properties has been attributed to this plant including nutritional agents, emetics, diuretics, diaphoretics, purgatives and pesticides. The objective of this study was assessing the in vitro nematocidal activity of a hydroalcoholic extract (HA-E) obtained from Ipomoea pauciflora (Cazahuate) flowers against Hc infective larvae (L3) and to identify its phytochemical profile (PhC-P). The assay was carried out using microtiter plates (MTP). Four HA-E concentrations were assessed and Ivermectin and distilled water were used as positive and negative control groups, respectively. Approximately 100 Hc L3 were deposited in each well (n=12) and incubated at 25-35°C for 7 days. Data were analyzed using ANOVA and a General Linear Model (GLM) followed by Tukey test (P<0.05). The treatments showing a concentration-dependent effect (CDE) were analyzed to identify their 50% and 90% lethal concentrations (CL50, 90) via a Probit Analysis. The highest mortality was observed at 50 mg/mL (82.64 ± 0.71%) and the lowest at 6.25 mg/mL (56.46 ± 2.49%), showing a CDE with increasing mortality from 6.25 to 50 mg/mL. The PhC-P revealed the presence of alkaloids, coumarins, flavonoids, tannins and triterpenes/ sterols. A HA-E from flowers of I. pauciflora will be considered to assess its potential use in the control of haemonchosis in small ruminants.
捻转血矛线虫（Hc）是一种吸血寄生虫，影响羊群的健康和生产力。使用化学驱虫药物（AH）进行驱虫是常见的方法；然而，这会导致寄生虫对 AH 的耐药性，并且由于牛奶、肉和副产物中的药物残留，这也构成了公共卫生风险。人们正在从植物中探索天然化合物，以减少这种寄生虫病，改善其健康和生产力，而没有 AH 的负面影响。番薯属是旋花科的一组攀援植物，具有多年生叶子和块根。该植物具有营养剂、催吐剂、利尿剂、发汗剂、泻药和杀虫剂等药用特性。本研究的目的是评估从Ipomoea pauciflora（Cazahuate）花中获得的水醇提取物（HA-E）对捻转血矛线虫感染性幼虫（L3）的体外杀线虫活性，并鉴定其植物化学特征（PhC-P）。该测定使用微量滴定板（MTP）进行。评估了 4 种 HA-E 浓度，伊维菌素和蒸馏水分别用作阳性和阴性对照组。每个孔（n=12）中大约放置 100 个 Hc L3，并在 25-35°C 下孵育 7 天。使用方差分析和广义线性模型（GLM）分析数据，然后使用 Tukey 检验（P<0.05）。对表现出浓度依赖性效应（CDE）的处理进行分析，通过概率分析确定其 50%和 90%致死浓度（CL50、90）。在 50mg/mL 时观察到最高死亡率（82.64 ± 0.71%），在 6.25mg/mL 时最低（56.46 ± 2.49%），表现出随着死亡率从 6.25 增加到 50mg/mL 的 CDE。PhC-P 显示存在生物碱、香豆素、类黄酮、单宁和三萜/甾醇。将从 I. pauciflora 花中获得的 HA-E 进行评估，以评估其在控制小反刍动物捻转血矛线虫病中的潜在用途。
