College of Chemistry and Material Science, Sichuan Normal University, Chengdu, Sichuan 610068, China.
College of Chemistry and Material Science, Sichuan Normal University, Chengdu, Sichuan 610068, China.
Food Chem. 2023 Nov 15;426:136659. doi: 10.1016/j.foodchem.2023.136659. Epub 2023 Jun 17.
Excessive intake of SO, a widely-used food additive, is able to cause respiratory, cardiovascular and neurological disease. For effectively monitoring SO level, we have developed a headspace single drop microextraction based visual colorimetry for highly sensitive and selective sensing of SO with TMB (3,3',5,5'-tetramethylbenzidine) as a classic chromogenic reagent. A combination of single drop and headspace microextraction integrated merits of high extraction efficiency, low consumption of reagents and excellent matrix interference-resistant ability. The colorimetric principle was based on oxidation of TMB, and SO could compete with TMB to preferentially react with ·OH, resulting in the fading of color blue that could be easily read out by naked eye. LOD was calculated to be 0.53 μM and 5 μM by UV-vis and naked eye, respectively. The method was successfully utilized to analysis of food samples, and the experimental device was miniaturized and easy to construct, thus showing a promising potential in field analysis.
过量摄入作为一种广泛使用的食品添加剂的 SO，能够导致呼吸道、心血管和神经系统疾病。为了有效地监测 SO 水平，我们开发了一种基于顶空单滴微萃取的可视化比色法，以 TMB（3,3',5,5'-四甲基联苯胺）作为经典显色试剂，对 SO 进行高灵敏度和选择性的传感。单滴和顶空微萃取的结合结合了高萃取效率、试剂消耗低和出色的基质干扰抗性的优点。比色原理基于 TMB 的氧化，SO 能够与 TMB 竞争，优先与·OH 反应，导致蓝色颜色褪色，可通过肉眼轻松读出。通过紫外可见分光光度法和肉眼分别计算出的 LOD 为 0.53 μM 和 5 μM。该方法成功地用于食品样品的分析，并且实验装置小型化且易于构建，因此在现场分析中显示出有前途的潜力。
