Center for Microorganisms' Investigation, Department of Microbiology and Parasitology, Biomedical Institute, Fluminense Federal University, Niterói, 24020-141 Rio de Janeiro, Brazil; Postgraduate Program in Applied Microbiology and Parasitology, Biomedical Institute, Fluminense Federal University, Niterói, 24210-130 Rio de Janeiro, Brazil.
Postgraduate Program in Applied Microbiology and Parasitology, Biomedical Institute, Fluminense Federal University, Niterói, 24210-130 Rio de Janeiro, Brazil; Postgraduate Program in Health Sciences, Federal University of Amapá (UNIFAP), Macapá 68903-419, Amapá, Brazil.
Cytokine. 2023 Sep;169:156278. doi: 10.1016/j.cyto.2023.156278. Epub 2023 Jun 23.
The innate immune response plays an important role during malaria. Toll-like receptors (TLR) are capable of recognizing pathogen molecules. We aimed to evaluate five polymorphisms in TLR-4, TLR-6, and TLR-9 genes and their association with cytokine levels and clinical parameters in malaria from the Brazil-French Guiana border.
A case-control study was conducted in Amapá, Brazil. P. vivax patients and individuals not infected were evaluated. Genotyping of five SNPs was carried out by qPCR. Circulating cytokines were measured by CBA. The MSP-1 IgG antibodies were performed by ELISA.
An association between TLR4 A299G with parasitemia was observed. There was an increase for IFN-ɤ, TNF-ɑ, IL-6, and IL-10 in the TLR-4 A299G and T3911, TLR-6 S249P, and TLR-9 1486C/T, SNPs for the studied malarial groups. There were significant findings for the TLR-4 variants A299G and T3911, TLR-9 1237C/T, and 1486C/T. For the reactivity of MSP-1 antibodies levels, no significant results were found in malaria, and control groups.
The profile of the immune response observed by polymorphisms in TLRs genes does not seem to be standard for all types of malaria infection around the world. This can depend on the human population and the species of Plasmodium.
先天免疫反应在疟疾中起着重要作用。 Toll 样受体 (TLR) 能够识别病原体分子。我们旨在评估 TLR-4、TLR-6 和 TLR-9 基因中的五个多态性及其与巴西-法属圭亚那边境地区疟疾的细胞因子水平和临床参数的关系。
在巴西阿马帕州进行了病例对照研究。评估了间日疟原虫患者和未感染者。通过 qPCR 进行了五个 SNP 的基因分型。通过 CBA 测量循环细胞因子。通过 ELISA 进行 MSP-1 IgG 抗体检测。
TLR4 A299G 与寄生虫血症之间存在关联。TLR-4 A299G 和 T3911、TLR-6 S249P 和 TLR-9 1486C/T 中的 SNP 观察到 IFN-ɤ、TNF-ɑ、IL-6 和 IL-10 增加。TLR-4 变体 A299G 和 T3911、TLR-9 1237C/T 和 1486C/T 存在显著发现。对于 MSP-1 抗体水平的反应性，在疟疾和对照组中均未发现显著结果。
TLR 基因多态性观察到的免疫反应谱似乎不适用于世界各地所有类型的疟疾感染。这可能取决于人类群体和疟原虫的种类。
