质谱单细胞代谢组学：准备好迎接黄金时代了吗？

Single-cell metabolomics by mass spectrometry: ready for primetime?

机构信息

Metabolomics and Analytics Center, Leiden Academic Centre of Drug Research, Leiden University, Leiden, the Netherlands.

Division of Drug Discovery and Safety, Leiden Academic Centre of Drug Research, Leiden University, Leiden, the Netherlands.

出版信息

Curr Opin Biotechnol. 2023 Aug;82:102963. doi: 10.1016/j.copbio.2023.102963. Epub 2023 Jun 23.

DOI:10.1016/j.copbio.2023.102963

PMID:37356380

Abstract

Single-cell metabolomics (SCMs) is a powerful tool for studying cellular heterogeneity by providing insight into the differences between individual cells. With the development of a set of promising SCMs pipelines, this maturing technology is expected to be widely used in biomedical research. However, before SCMs is ready for primetime, there are some challenges to overcome. In this review, we summarize the trends and challenges in the development of SCMs. We also highlight the latest methodologies, applications, and sketch the perspective for integration with other omics and imaging approaches.

摘要

单细胞代谢组学（SCMs）是一种强大的工具，通过深入了解单个细胞之间的差异，为研究细胞异质性提供了线索。随着一组有前途的 SCMs 管道的发展，这项成熟的技术有望在生物医学研究中得到广泛应用。然而，在 SCMs 准备好大放异彩之前，还有一些挑战需要克服。在这篇综述中，我们总结了 SCMs 发展的趋势和挑战。我们还强调了最新的方法学、应用，并勾勒出与其他组学和成像方法相结合的前景。