Qi Jiahui, Wang Hui, Wu Xinyi, Noman Muhammad, Wen Ya, Li Dayong, Song Fengming
Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou, Zhejiang, 310058, China; Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Biotechnology, Zhejiang University, Hangzhou, Zhejiang, 310058, China; State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, Zhejiang, 310058, China; Institute of Aging, Key Laboratory of Alzheimer's Disease of Zhejiang Province, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China.
Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou, Zhejiang, 310058, China; Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Biotechnology, Zhejiang University, Hangzhou, Zhejiang, 310058, China; State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, Zhejiang, 310058, China.
Plant Physiol Biochem. 2023 Aug;201:107854. doi: 10.1016/j.plaphy.2023.107854. Epub 2023 Jun 20.
Plant AT-rich sequence and zinc-binding (PLATZ) proteins are plant-specific transcription factors involved in growth, development, and stress responses. Here, we conducted a genome-wide characterization of the watermelon ClPLATZ family and examined its expression responsiveness to defense hormones and pathogen infection along with putative functions in biotic and abiotic stress responses. The watermelon genome contains 12 putative ClPLATZ genes, encoding proteins with a characteristic PLATZ domain, and their promoters contain various cis-elements related to plant growth, development, phytohormones and stress response. The ClPLATZ genes, except ClPLATZ6, are differentially expressed in response to defense hormones (e.g., salicylic acid and methyl jasmonate) and fungal infections caused by Fusarium oxysporum f. sp. niveum and Stagonosporopsis cucurbitacearum. Most ClPLATZ proteins interact with other proteins (viz., ClDP, ClRPT2a, and ClRPC53). Among ClPLATZ proteins, ClPLATZ8, 9, 10, and 11 are predominately localized in the nucleus. ClPLATZ3 and 8 positively, but ClPLATZ11 negatively regulate resistance against Pseudomonas syringe pv. tomato DC3000 in transgenic Arabidopsis lines. ClPLATZ8 and 11 positively regulate stress tolerance to NaCl and mannitol during seed germination in transgenic Arabidopsis. In conclusion, the characterization of the ClPLATZ family provides insights into the biological functions of ClPLATZ genes in growth, development, and stress response in watermelon. Further, the involvement of certain ClPLATZ genes in biotic and abiotic stress response in transgenic Arabidopsis suggests their potential application in engineering stress-tolerant crops.
植物富含AT序列和锌结合（PLATZ）蛋白是参与生长、发育和应激反应的植物特异性转录因子。在此，我们对西瓜ClPLATZ家族进行了全基因组特征分析，并研究了其对防御激素和病原体感染的表达响应以及在生物和非生物应激反应中的假定功能。西瓜基因组包含12个假定的ClPLATZ基因，编码具有特征性PLATZ结构域的蛋白质，其启动子包含与植物生长、发育、植物激素和应激反应相关的各种顺式元件。除ClPLATZ6外，ClPLATZ基因在响应防御激素（如水杨酸和茉莉酸甲酯）以及尖孢镰刀菌西瓜专化型和葫芦科刺盘孢引起的真菌感染时差异表达。大多数ClPLATZ蛋白与其他蛋白（即ClDP、ClRPT2a和ClRPC53）相互作用。在ClPLATZ蛋白中，ClPLATZ8、9、10和11主要定位于细胞核。在转基因拟南芥系中，ClPLATZ3和8正向调节对番茄丁香假单胞菌番茄致病变种DC3000的抗性，但ClPLATZ11负向调节。在转基因拟南芥种子萌发期间，ClPLATZ8和11正向调节对NaCl和甘露醇的胁迫耐受性。总之，ClPLATZ家族的特征分析为ClPLATZ基因在西瓜生长、发育和应激反应中的生物学功能提供了见解。此外，某些ClPLATZ基因参与转基因拟南芥的生物和非生物应激反应，表明它们在工程抗逆作物中的潜在应用。
