紫贻贝中父系遗传线粒体DNA的雄性特异性开放阅读框作为蛋白质编码基因的验证。

Validation of the male-specific ORF of the paternally-transmitted mtDNA in Mytilus edulis as a protein-coding gene.

作者信息

Debelli Alizée, Kienzle Laura, Khorami Hajar Hosseini, Angers Annie, Breton Sophie

机构信息

Département de Sciences Biologiques, Université de Montréal, Montréal, QC H3C 3J7, Canada.

出版信息

Gene. 2023 Aug 30;879:147586. doi: 10.1016/j.gene.2023.147586. Epub 2023 Jun 24.

DOI:10.1016/j.gene.2023.147586

PMID:37356740

Abstract

There appears to be an additional set of sex-specific mtDNA-encoded proteins in bivalve species with doubly uniparental mitochondrial inheritance that may be involved in the transmission of the female and male mitogenomes. In the marine mussel Mytilus edulis, the translation of the female-specific open reading frame (F-ORF) was demonstrated but the translation of the male-specific ORF (M-ORF) remains to be shown. Here we validate the male-specific ORF of the paternal mitogenome in M. edulis as a protein-coding gene. The M-ORF protein was detected only in male gonads and localized in sperm mitochondria and acrosome, suggesting that it is involved in a key sperm function in Mytilus edulis.

摘要

在具有双亲单亲线粒体遗传的双壳贝类物种中，似乎存在另一组性别特异性的线粒体DNA编码蛋白，它们可能参与雌性和雄性线粒体基因组的传递。在海洋贻贝紫贻贝中，已证实了雌性特异性开放阅读框（F-ORF）的翻译，但雄性特异性开放阅读框（M-ORF）的翻译仍有待证实。在此，我们验证了紫贻贝父本线粒体基因组中的雄性特异性开放阅读框作为一个蛋白质编码基因。仅在雄性性腺中检测到M-ORF蛋白，且其定位于精子线粒体和顶体中，这表明它参与了紫贻贝精子的关键功能。