Sutherland B, Stewart D, Kenchington E R, Zouros E
Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada.
Genetics. 1998 Jan;148(1):341-7. doi: 10.1093/genetics/148.1.341.
Species of the marine mussel family Mytilidae have two types of mitochondrial DNA: one that is transmitted from the mother to both female and male offspring (the F type) and one that is transmitted from the father to sons only (the M type). By using pair matings that produce only female offspring or a mixture of female and male offspring and a pair of oligonucleotide primers that amplify part of the COIII gene of the M but not the F mitochondrial genome, we demonstrate that both male and female embryos receive M mtDNA through the sperm and that within 24 hr after fertilization the M mtDNA is eliminated or is drastically reduced in female embryos but maintained in male embryos. These observations are important for understanding the relationship between mtDNA transmission and sex determination in species with doubly uniparental inheritance of mitochondrial DNA.
海洋贻贝科的物种有两种类型的线粒体DNA：一种是从母亲传递给雌性和雄性后代（F型），另一种是仅从父亲传递给儿子（M型）。通过使用只产生雌性后代或雌性和雄性后代混合的配对交配，以及一对能扩增M型而非F型线粒体基因组COIII基因部分的寡核苷酸引物，我们证明了雄性和雌性胚胎都通过精子获得M型线粒体DNA，并且在受精后24小时内，M型线粒体DNA在雌性胚胎中被消除或大幅减少，但在雄性胚胎中得以保留。这些观察结果对于理解线粒体DNA单亲双系遗传物种中线粒体DNA传递与性别决定之间的关系具有重要意义。
