Institute of Biological Sciences, Federal University of Minas Gerais, 31270-901, Belo Horizonte, MG, Brazil,
Department of Biological Sciences, State University of Santa Cruz, Campus Soane Nazaré de Andrade, 45662-900, Ilhéus, BA, Brazil.
J Avian Med Surg. 2023 Jun;37(1):32-40. doi: 10.1647/21-00075.
Avian chlamydiosis is a disease that occurs in birds, especially parrots, and is caused by the Gram-negative bacterium . Wild Animal Screening Centers in Brazil receive, maintain, treat, and place (preferably to nature) wild animals recovered from illegal trafficking. We performed molecular testing for avian chlamydiosis in parrots from the genus that were presented to these centers. Cloacal swab samples were collected from 59 parrots ( species) and transported in aqueous or culture medium. The samples were subsequently submitted for DNA extraction by the boiling method, polymerase chain reaction (PCR) amplification using CPF/CPR primers, and agarose gel electrophoresis. Conjunctivitis, nasal discharge, and poor body condition were the clinical signs associated with a differential disease diagnosis of avian chlamydiosis. Transport medium did not have an effect on the test results. The prevalence of in the samples was 37% (22/59, 95% confidence interval: 25-49). There was a significant ( = 0.009) association between the PCR test results and clinical signs. Follow-up testing was conducted on a subgroup of 14 individuals that initially tested negative on PCR; 50% (7/14) of these birds were found to be positive within 24 days of the first test. The results of this study confirm the feasibility of using the CPF/CFP primer-based PCR to detect in species, describe a less costly method of transporting biological material for DNA extraction, and evaluate the temporal aspect for obtaining positive results through molecular testing for in species.
禽衣原体病是一种发生在鸟类，特别是鹦鹉身上的疾病，由革兰氏阴性菌引起。巴西的野生动物筛查中心接收、维持、治疗和安置（最好是在自然环境中）从非法交易中回收的野生动物。我们对这些中心送来的属鹦鹉进行了禽衣原体病的分子检测。从 59 只鹦鹉（种）采集了泄殖腔拭子样本，并在水性或培养基中运输。随后通过煮沸法提取 DNA，使用 CPF/CPR 引物进行聚合酶链反应（PCR）扩增，并进行琼脂糖凝胶电泳。结膜炎、鼻分泌物和身体状况不佳是与禽衣原体病鉴别诊断相关的临床症状。运输培养基对检测结果没有影响。样本中 的患病率为 37%（22/59，95%置信区间：25-49）。PCR 检测结果与临床症状之间存在显著关联（=0.009）。对最初 PCR 检测呈阴性的 14 只鸟的亚组进行了后续检测；其中 50%（7/14）在首次检测后 24 天内呈阳性。这项研究的结果证实了使用基于 CPF/CFP 引物的 PCR 检测属种中的 的可行性，描述了一种成本更低的运输生物材料进行 DNA 提取的方法，并评估了通过分子检测属种中 的时间方面。
