Department of Pediatric Intensive Care Unit, Shanghai Children's Medical Center, School of Medicine, Shanghai Jiaotong University.
Department of Thoracic and Cardiovascular Surgery, Shanghai Children's Hospital, School of Medicine, Shanghai Jiaotong University.
J Vis Exp. 2023 Jun 9(196). doi: 10.3791/65372.
Right ventricular (RV) volume overload (VO) is common in children with congenital heart disease. In view of distinct developmental stages,the RV myocardium may respond differently to VO in children compared to adults. The present study aims to establish a postnatal RV VO model in mice using a modified abdominal arteriovenous fistula. To confirm the creation of VO and the following morphological and hemodynamic changes of the RV, abdominal ultrasound, echocardiography, and histochemical staining were performed for 3 months. As a result, the procedure in postnatal mice showed an acceptable survival and fistula success rate. In VO mice, the RV cavity was enlarged with a thickened free wall, and the stroke volume was increased by about 30%-40% within 2 months after surgery. Thereafter, the RV systolic pressure increased, corresponding pulmonary valve regurgitation was observed, and small pulmonary artery remodeling appeared. In conclusion, modified arteriovenous fistula (AVF) surgery is feasible to establish the RV VO model in postnatal mice. Considering the probability of fistula closure and elevated pulmonary artery resistance, abdominal ultrasound and echocardiography must be performed to confirm the model status before application.
右心室（RV）容量超负荷（VO）在先天性心脏病患儿中很常见。鉴于不同的发育阶段，与成人相比，儿童的 RV 心肌可能对 VO 有不同的反应。本研究旨在使用改良的腹动静脉瘘在小鼠中建立 RV VO 模型。为了确认 VO 的建立以及 RV 的后续形态和血液动力学变化，对其进行了 3 个月的腹部超声、超声心动图和组织化学染色。结果显示，该术后程序在新生小鼠中具有可接受的存活率和瘘管成功率。在 VO 小鼠中，RV 腔扩大，游离壁增厚，术后 2 个月内每搏量增加约 30%-40%。此后，RV 收缩压升高，出现相应的肺动脉瓣反流，以及小肺动脉重塑。总之，改良的动静脉瘘（AVF）手术可在新生小鼠中建立 RV VO 模型。考虑到瘘管闭合和肺动脉阻力升高的可能性，在应用前必须进行腹部超声和超声心动图检查以确认模型状态。
