Liaocheng University, Liaocheng, 252000, China.
Liaocheng Land and Resources Bureau, Liaocheng, 252000, China.
Curr Microbiol. 2023 Jun 26;80(8):259. doi: 10.1007/s00284-023-03296-y.
Trichoderma longibrachiatum SMF2 is an important biocontrol strain isolated by our group that can promote plant growth and induce plant disease resistance. To further study its biocontrol mechanism, the effector proteins secreted by T. longibrachiatum SMF2 were analyzed through bioinformatics and transcriptome sequencing. Overall, 478 secretory proteins produced by T. longibrachiatum were identified, of which 272 were upregulated after treatment with plants. Functional annotation showed that 36 secretory proteins were homologous with different groups of effectors from pathogenic microorganisms. Moreover, the quantitative PCR results of six putative effector proteins were consistent with those of transcriptome sequencing. Taken together, these findings indicate that the secretory proteins secreted by T. longibrachiatum SMF2 may act as effectors to facilitate its own growth and colonization or to induce plant immunity response.
长枝木霉 SMF2 是本课题组分离得到的一种重要生防菌株，能够促进植物生长并诱导植物抗病性。为了进一步研究其生防机制，我们通过生物信息学和转录组测序分析了长枝木霉 SMF2 分泌的效应蛋白。总体上，鉴定出长枝木霉分泌的 478 种分泌蛋白，其中 272 种在与植物共培养后上调。功能注释表明，36 种分泌蛋白与来自病原微生物的不同组别的效应子具有同源性。此外，6 种假定效应蛋白的定量 PCR 结果与转录组测序结果一致。综上所述，这些发现表明长枝木霉 SMF2 分泌的分泌蛋白可能作为效应子促进其自身的生长和定殖，或诱导植物的免疫反应。
