Department of Endocrinology, Institute of Geriatric Medicine, Liyuan Hospital, Tongji Medical College, Huazhong University of Science and Technology, 39 Lake Road, East Lake Ecological Scenic, Wuhan, 430077, Hubei Province, China.
Department of Laboratory Medicine, Liyuan Hospital, Tongji Medical College, Huazhong University of Science and Technology, 39 Lake Road, East Lake Ecological Scenic, Wuhan, 430077, Hubei Province, China.
J Endocrinol Invest. 2024 Jan;47(1):201-212. doi: 10.1007/s40618-023-02137-w. Epub 2023 Jun 26.
Mounting evidence has shown that caveolin-1 plays a pathological role in the progression of albuminuria. Our study aimed to provide clinical evidence showing whether circulating caveolin-1 levels were associated with microalbuminuria (MAU) in women with overt diabetes mellitus in pregnancy (ODMIP).
A total of 150 pregnant women were enrolled in different groups, including 40 women with ODMIP and MAU (ODMIP + MAU), 40 women with ODMIP, and 70 women without ODMIP (Non-ODMIP). Plasma caveolin-1 levels were determined by ELISA. The presence of caveolin-1 in the human umbilical vein vascular wall was evaluated by immunohistochemical and western blot analysis, respectively. Albumin transcytosis across endothelial cells was measured using an established nonradioactive in vitro approach.
Significantly increased levels of plasma caveolin-1 were detected in ODMIP + MAU women. The Pearson's correlation analysis revealed a positive correlation between plasma caveolin-1 levels and Hemoglobin A1c (HbA1c %) as well as with MAU in the ODMIP + MAU group. Simultaneously, experimental knockdown or overexpression of caveolin-1 significantly decreased or increased the level of albumin transcytosis across both human and mouse glomerular endothelial cells (GECs), respectively.
Our data showed a positive association between plasma caveolin-1 levels and microalbuminuria in ODMIP + MAU.
越来越多的证据表明，窖蛋白-1 在白蛋白尿的进展中起着病理性作用。我们的研究旨在提供临床证据，表明显性糖尿病合并妊娠（ODMIP）女性的循环窖蛋白-1 水平是否与微量白蛋白尿（MAU）相关。
共纳入 150 名孕妇，分为 4 组，包括 40 名 ODMIP 合并 MAU（ODMIP+MAU）患者、40 名 ODMIP 患者和 70 名无 ODMIP（非 ODMIP）患者。通过 ELISA 测定血浆窖蛋白-1 水平。通过免疫组织化学和 Western blot 分析分别评估人脐静脉血管壁中窖蛋白-1 的存在。使用已建立的非放射性体外方法测量白蛋白跨内皮细胞转胞作用。
在 ODMIP+MAU 女性中检测到血浆窖蛋白-1 水平显著升高。Pearson 相关分析显示，ODMIP+MAU 组中血浆窖蛋白-1 水平与血红蛋白 A1c（HbA1c%）和 MAU 呈正相关。同时，实验性敲低或过表达窖蛋白-1 分别显著降低或增加了人肾小球内皮细胞（GEC）和小鼠 GEC 中白蛋白跨内皮细胞转胞作用的水平。
我们的数据显示，ODMIP+MAU 患者的血浆窖蛋白-1 水平与微量白蛋白尿之间存在正相关。
