Department of Endocrinology, Pingyang Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325400, China.
School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, 325035, China.
Metab Brain Dis. 2023 Oct;38(7):2281-2288. doi: 10.1007/s11011-023-01255-x. Epub 2023 Jun 26.
Diabetes has been reported to induce brain metabolic disturbance, but the effect of transient neonatal hyperglycemia (TNH) on brain metabolism remains unclear. Herein the rats were treated with a single intraperitoneal injection of 100 µg/g body weight of streptozotocin within 12 h after birth and displayed a typical clinical characteristic of TNH. Then we used NMR-based metabolomics to examine the metabolic changes in the hippocampus between TNH and normal control (Ctrl) rats at postnatal 7 days (P7) and 21 days (P21). The results show that TNH rats had significantly increased levels of N-acetyl aspartate, glutamine, aspartate and choline in the hippocampus relative to Ctrl rats at P7. Moreover, we found that the levels of alanine, myo-inositol and choline were significantly lower in TNH rats, although their blood glucose levels have been recovered to the normal level at P21. Therefore, our results suggest that TNH may have a long-term effect on hippocampal metabolic changes mainly involving neurotransmitter metabolism and choline metabolism.
糖尿病已被报道可诱导脑代谢紊乱，但短暂性新生儿高血糖（TNH）对脑代谢的影响尚不清楚。本研究中，新生大鼠于出生后 12 小时内腹腔注射 100µg/g 体重的链脲佐菌素，以建立 TNH 模型，该模型具有典型的 TNH 临床特征。然后，我们使用基于 NMR 的代谢组学方法，在出生后 7 天（P7）和 21 天（P21）检测 TNH 大鼠和正常对照组（Ctrl）大鼠海马中的代谢变化。结果表明，与 Ctrl 大鼠相比，TNH 大鼠在 P7 时海马中 N-乙酰天冬氨酸、谷氨酰胺、天冬氨酸和胆碱的水平显著升高。此外，我们发现尽管 TNH 大鼠的血糖水平已恢复至正常水平，但在 P21 时，其丙氨酸、肌醇和胆碱的水平显著降低。因此，我们的结果表明，TNH 可能对海马代谢变化具有长期影响，主要涉及神经递质代谢和胆碱代谢。
