Garcia-Corredor Diego, Alvarado Mateo, Pulido-Medellín Martín, Muñoz Marina, Cruz-Saavedra Lissa, Hernández Carolina, Giraldo Julio Cesar, Vásquez-Arteaga Luis R, Morillo Coronado Ana Cruz, Ramírez Juan David
Grupo de Investigación en Medicina Veterinaria y Zootecnia (GIDIMEVETZ), Facultad de Ciencias Agropecuarias, Universidad Pedagógica y Tecnológica de Colombia (UPTC), Tunja, Colombia.
Centro de Investigaciones en Microbiología y Biotecnología-UR (CIMBIUR), Facultad de Ciencias Naturales, Universidad del Rosario, Bogotá, Colombia.
Front Vet Sci. 2023 Jun 9;10:1171147. doi: 10.3389/fvets.2023.1171147. eCollection 2023.
is a zoonotic trematode that affects a wide range of hosts, including cattle, sheep, and goats. The economic impact of the parasite on the cattle industry is significant, with high losses reported worldwide. While its impact on human health was previously underestimated, recent years have seen a rise in fascioliasis cases, leading to increased interest among researchers globally. To characterize the genetic diversity and intraspecific variation of this parasite in South America, specifically in Colombia, we collected 105 adult parasites from cattle bile ducts in seven Colombian departments (Antioquia, Boyacá, Santander, Cauca, Cundinamarca, Nariño, Norte de Santander, and Santander) to assess the parasite's phenotypic analyses, genetic diversity, and population structure. A computer image analysis system (CIAS) was applied based on standardized morphological measurements. Liver-fluke size was studied by principal component analysis (PCA). DNA sequences were obtained for nuclear markers such as the 28S, β-tubulin 3, ITS1, ITS2, and the mitochondrial marker Cytochrome Oxidase I (COI). Multiple statistical tests were performed, and the parasite's population structure was analyzed. Maximum Likelihood (ML) phylogenetic reconstructions were carried out using the sequences obtained herein and sequences available in GenBank. Morphological results revealed that all the obtained individuals matched 's morphology. There was no evidence of high genetic diversity, and the absence of genetic structure at the country-level was notable, possibly caused by a demographic expansion of this trematode in Colombia or the low resolution of the molecular markers employed. Future studies are still needed to unveil the genetic population structure of across the country.
是一种人畜共患吸虫，可感染包括牛、绵羊和山羊在内的多种宿主。该寄生虫对养牛业的经济影响巨大，全球范围内均有高额损失的报告。虽然其对人类健康的影响此前被低估，但近年来肝吸虫病病例有所增加，这引发了全球研究人员的更多关注。为了表征这种寄生虫在南美洲，特别是在哥伦比亚的遗传多样性和种内变异，我们从哥伦比亚七个省（安蒂奥基亚、博亚卡、桑坦德、考卡、昆迪纳马卡、纳里尼奥、北桑坦德和桑坦德）的牛胆管中收集了105条成年寄生虫，以评估该寄生虫的表型分析、遗传多样性和种群结构。基于标准化形态测量应用了计算机图像分析系统（CIAS）。通过主成分分析（PCA）研究肝吸虫大小。获得了核标记如28S、β-微管蛋白3、ITS1、ITS2以及线粒体标记细胞色素氧化酶I（COI）的DNA序列。进行了多项统计测试，并分析了寄生虫的种群结构。使用本文获得的序列和GenBank中可用的序列进行了最大似然（ML）系统发育重建。形态学结果表明，所有获得的个体均符合的形态。没有证据表明存在高遗传多样性，并且在国家层面不存在遗传结构这一点值得注意，这可能是由于该吸虫在哥伦比亚的种群扩张或所采用分子标记的低分辨率所致。仍需要进一步的研究来揭示该国的遗传种群结构。
