Molecular identification of and species isolates in ruminants livestock from Kashan and Arak in center of Iran.

and are important trematode infections that affect humans and ruminants worldwide. Molecular techniques have a conclusive role in detection of liver flukes. The purpose of the current study was to find outthe genotypic diversity of and spp. isolated from different hosts in Iran. Totally, 160 and 200 adult and spp. isolates were collected from infected cattle, sheep, and goatsfrom two abattoirs in the center of Iran.PCR-RFLP, and DNA sequences nuclear markers (18 S, 28 S, ITS) and the mitochondrial marker (ND1, CO1) were applied. PCR products of and samples were subjected to digestion by , , , , and enzymes. DNA from 60 isolates of and of different hosts were sequenced and evaluated. The PCR reaction showed the length of 18 S, 28 S, ND1, CO1 of at 260 bp, 618 bp, 700 bp, and 500 bp, and the length of the ITS2 and 28 S of was 236 bp and 963 bp respectively. has an RFLP pattern of 110, and 126 bp (ITS2), and 116, 293, 409 bp (28 S) using, and restriction enzymes. has a profile of 333, and 285 bp (28 S) using enzyme. The RFLP pattern of genotype . was 73, 120, and 507 bp (ND1) and 119 and 381 bp (CO1) in size using and enzymes. Using the PCR-RFLP, two species of ( and ), and one species of Dicrocoelium () were identified. To uncover the genetic population structure of liver flukes across the country, future studies are still required.