Arbabi Mohsen, Hooshyar Hossein, Delavari Mahdi
Department of Medical Parasitology, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran.
Infectious Diseases Research Center, Kashan University of Medical Sciences, Kashan, Islamic Republic of Iran.
J Parasit Dis. 2025 Jun;49(2):453-464. doi: 10.1007/s12639-024-01771-2. Epub 2024 Dec 26.
and are important trematode infections that affect humans and ruminants worldwide. Molecular techniques have a conclusive role in detection of liver flukes. The purpose of the current study was to find outthe genotypic diversity of and spp. isolated from different hosts in Iran. Totally, 160 and 200 adult and spp. isolates were collected from infected cattle, sheep, and goatsfrom two abattoirs in the center of Iran.PCR-RFLP, and DNA sequences nuclear markers (18 S, 28 S, ITS) and the mitochondrial marker (ND1, CO1) were applied. PCR products of and samples were subjected to digestion by , , , , and enzymes. DNA from 60 isolates of and of different hosts were sequenced and evaluated. The PCR reaction showed the length of 18 S, 28 S, ND1, CO1 of at 260 bp, 618 bp, 700 bp, and 500 bp, and the length of the ITS2 and 28 S of was 236 bp and 963 bp respectively. has an RFLP pattern of 110, and 126 bp (ITS2), and 116, 293, 409 bp (28 S) using, and restriction enzymes. has a profile of 333, and 285 bp (28 S) using enzyme. The RFLP pattern of genotype . was 73, 120, and 507 bp (ND1) and 119 and 381 bp (CO1) in size using and enzymes. Using the PCR-RFLP, two species of ( and ), and one species of Dicrocoelium () were identified. To uncover the genetic population structure of liver flukes across the country, future studies are still required.
[吸虫名称1]和[吸虫名称2]是影响全球人类和反刍动物的重要吸虫感染。分子技术在肝吸虫检测中具有决定性作用。本研究的目的是找出从伊朗不同宿主分离出的[吸虫名称1]和[吸虫名称2]物种的基因型多样性。总共从伊朗中部的两个屠宰场收集了160个和200个成年[吸虫名称1]和[吸虫名称2]分离株，这些分离株来自受感染的牛、绵羊和山羊。应用了PCR-RFLP以及DNA序列核标记（18S、28S、ITS）和线粒体标记（ND1、CO1）。[吸虫名称1]和[吸虫名称2]样本的PCR产物用[酶名称1]、[酶名称2]、[酶名称3]、[酶名称4]和[酶名称5]酶进行消化。对来自不同宿主的60个[吸虫名称1]和[吸虫名称2]分离株的DNA进行测序和评估。PCR反应显示[吸虫名称1]的18S、28S、ND1、CO1长度分别为260bp、618bp、700bp和500bp，[吸虫名称2]的ITS2和28S长度分别为236bp和963bp。使用[酶名称1]、[酶名称2]和[酶名称3]限制酶时，[吸虫名称1]的RFLP模式为110bp和126bp（ITS2），以及116bp、293bp、409bp（28S）。使用[酶名称4]酶时，[吸虫名称2]的图谱为333bp和285bp（28S）。基因型[基因型名称]的RFLP模式使用[酶名称5]和[酶名称6]酶时，大小分别为73bp、120bp和507bp（ND1）以及119bp和381bp（CO1）。使用PCR-RFLP鉴定出两种[吸虫名称1]（[具体种类1]和[具体种类2]）和一种双腔吸虫（[双腔吸虫具体种类]）。为了揭示全国肝吸虫的遗传种群结构，仍需要进一步的研究。
