Schor A M, Schor S L
Microvasc Res. 1986 Jul;32(1):21-38. doi: 10.1016/0026-2862(86)90041-5.
Endothelial cells (BREC) and pericytes (BRP) were isolated from the bovine retinal microvasculature. These cells were first identified by morphological criteria and by their differential staining for Factor VIII related antigen. BREC and BRP responded differently to a number of experimental parameters in vitro; for example, the plating efficiency of BREC was enhanced by the use of a gelatin substratum and medium conditioned by either endothelial cells or pericytes; oxygen tension had no effect. In contrast, the plating efficiency of BRP was only enhanced by low oxygen tension. Conditioned media also stimulated the proliferation of BREC, but not that of BRP. The saturation density reached by BREC was dependent on the initial plating density while BRP plated at different initial densities reached the same final density. The in vitro behavior of the retinal microvascular cells was also compared to that of large vessel (aorta) endothelial cells (BAEC) and smooth muscle cells (SMC). Aortic and retinal endothelial cells showed similar morphology and behavior. When initially plated as a homogeneous cell suspension within a collagen matrix, both BREC and BAEC self-associated to form three-dimensional meshworks; this morphogenesis was accomplished by cell migration and did not involve cell proliferation. By contrast, BRP and SMC divided and remained homogeneously distributed when plated within a collagen gel matrix. BRP and SMC did, however, behave differently when plated on the surface of a collagen gel; SMC migrated extensively into the gel while BRP remained confined to the gel surface. BRP grown on any substratum began to retract upon themselves shortly after confluence, producing characteristic nodules interconnected by cellular strands. BRP and SMC were able to contract a collagen gel substratum, while retinal and aortic endothelial cells were unable to do so. These results provide new means for the in vitro characterization of endothelial cells, smooth muscle cells and pericytes.
内皮细胞（BREC）和周细胞（BRP）是从牛视网膜微血管中分离出来的。这些细胞首先通过形态学标准以及它们对VIII因子相关抗原的差异染色来鉴定。BREC和BRP在体外对许多实验参数的反应不同；例如，使用明胶基质和内皮细胞或周细胞条件培养基可提高BREC的接种效率；氧张力没有影响。相比之下，只有低氧张力才能提高BRP的接种效率。条件培养基也刺激BREC的增殖，但不刺激BRP的增殖。BREC达到的饱和密度取决于初始接种密度，而不同初始密度接种的BRP达到相同的最终密度。还将视网膜微血管细胞的体外行为与大血管（主动脉）内皮细胞（BAEC）和平滑肌细胞（SMC）的行为进行了比较。主动脉和视网膜内皮细胞表现出相似的形态和行为。当最初作为均匀的细胞悬液接种在胶原基质中时，BREC和BAEC都会自我聚集形成三维网络；这种形态发生是通过细胞迁移完成的，不涉及细胞增殖。相比之下，BRP和SMC接种在胶原凝胶基质中时会分裂并保持均匀分布。然而，当接种在胶原凝胶表面时，BRP和SMC的行为有所不同；SMC广泛迁移到凝胶中，而BRP则局限于凝胶表面。在任何基质上生长的BRP在汇合后不久就开始自行收缩，产生由细胞条索相互连接的特征性结节。BRP和SMC能够收缩胶原凝胶基质，而视网膜和主动脉内皮细胞则不能。这些结果为内皮细胞、平滑肌细胞和周细胞在体外的特性鉴定提供了新的方法。
