Saba Amal I, Elbakary Reda H, Afifi Omayma K, Sharaf Eldin Heba E M
Histology and Cell Biology Department, Faculty of Medicine, Tanta University, Tanta 31527, Egypt.
Diseases. 2023 Jun 9;11(2):84. doi: 10.3390/diseases11020084.
Oxymetholone is one of the anabolic steroids that has widely been used among teenagers and athletes to increase their muscle bulk. It has undesirable effects on male health and fertility. In this study, the therapeutic effects of platelet-rich plasma (PRP) on oxymetholone-induced testicular toxicity were investigated in adult albino rats. During the experiments, 49 adult male albino rats were divided into 4 main groups: Group 0 (donor group) included 10 rats for the donation of PRP, Group I (control group) included 15 rats, Group II included 8 rats that received 10 mg/kg of oxymetholone orally, once daily, for 30 days, and Group III included 16 rats and was subdivided into 2 subgroups (IIIa and IIIb) that received oxymetholone the same as group II and then received PRP once and twice, respectively. Testicular tissues of all examined rats were obtained for processing and histological examination and sperm smears were stained and examined for sperm morphology. Oxymetholone-treated rats revealed wide spaces in between the tubules, vacuolated cytoplasm, and dark pyknotic nuclei of most cells, as well as deposition of homogenous acidophilic material between the tubules. Electron microscopic examination showed vacuolated cytoplasm of most cells, swollen mitochondria, and perinuclear dilatation. Concerning subgroup IIIa (PRP once), there was a partial improvement in the form of decreased vacuolations and regeneration of spermatogenic cells, as well as a reasonable improvement in sperm morphology. Regarding subgroup IIIb (PRP twice), histological sections revealed restoration of the normal testicular structure to a great extent, regeneration of the spermatogenic cells, and most sperms had normal morphology. Thus, it is recommended to use PRP to minimize structural changes in the testis of adult albino rats caused by oxymetholone.
羟甲烯龙是一种合成代谢类固醇，在青少年和运动员中被广泛使用以增加肌肉量。它对男性健康和生育能力有不良影响。在本研究中，研究了富血小板血浆（PRP）对羟甲烯龙诱导的成年白化大鼠睾丸毒性的治疗作用。实验期间，将49只成年雄性白化大鼠分为4个主要组：第0组（供体组）包括10只用于捐献PRP的大鼠，第I组（对照组）包括15只大鼠，第II组包括8只大鼠，它们每天口服10 mg/kg羟甲烯龙，持续30天，第III组包括16只大鼠，并细分为2个亚组（IIIa和IIIb），它们与第II组一样接受羟甲烯龙，然后分别接受一次和两次PRP。获取所有检查大鼠的睾丸组织进行处理和组织学检查，并对精子涂片进行染色并检查精子形态。经羟甲烯龙处理的大鼠显示小管之间有宽大间隙、细胞质空泡化、大多数细胞的深色固缩核，以及小管之间有均匀嗜酸性物质沉积。电子显微镜检查显示大多数细胞的细胞质空泡化、线粒体肿胀和核周扩张。关于IIIa亚组（一次PRP），出现了部分改善，表现为空泡化减少和生精细胞再生，精子形态也有合理改善。关于IIIb亚组（两次PRP），组织学切片显示睾丸结构在很大程度上恢复正常，生精细胞再生，大多数精子形态正常。因此，建议使用PRP以尽量减少成年白化大鼠睾丸因羟甲烯龙引起的结构变化。
