Department of Surgery, Anesthesiology, and Radiology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt.
Department of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt.
Life Sci. 2020 Sep 1;256:117982. doi: 10.1016/j.lfs.2020.117982. Epub 2020 Jun 17.
This study was designed to evaluate the protective and therapeutic efficacy of platelet-rich plasma (PRP) against testicular degeneration and germ cell apoptosis after induced spermatic cord torsion/detorsion (TD) in rats.
Forty rats were allocated into 5 groups: 1) control, 2) short torsion/detorsion (STD), 3) long torsion detorsion (LTD), 4) protective (PRP/P) and 5) treatment (PRP/T). Testicular ischemia was induced by twisting the right testis 1080° clockwise for 2.5 h. PRP (10 μl) was injected intra-testicular 5 min before (PRP/P) and 3 h after (PRP/T) detorsion. At the end of the experiment, rats were euthanized at 2, 30, 2, and 30 days for groups 2-5 respectively. Nitric oxide, malondialdehyde, catalase, total antioxidant capacity, reduced glutathione, glutathione-S-transferase, interleukin1 beta, tumor necrosis factor, caspase-3, and B-cell lymphoma 2 expressions were assessed in the testes. Moreover, histological examination was performed.
PRP treatment significantly mitigated the torsion-detorsion induced testicular degeneration. Particularly, by improving the state of oxidative stress (NO, P = 0.0001) and antioxidant markers (TAC, GSH, GST, P = 0.0001-0.01) and decreasing the expression of IL-1β, TNF-α and cas 3 and increase the BCL2 fold changes (P = 0.0001). The protective use of PRP is superior to the therapeutic use of PRP in the restoration of the testicular histoarchitecture following TD.
This study illustrates the cyto-protective role of PRP against TD induced testicular cell injury that highlight possible application of PRP as a complementary therapy in different testicular degenerative diseases which might attribute to its ability to ameliorate the oxidative stress and inhibit induced apoptosis.
本研究旨在评估富血小板血浆（PRP）对大鼠精索扭转/复位（TD）后睾丸退化和生殖细胞凋亡的保护和治疗作用。
将 40 只大鼠分为 5 组：1）对照组，2）短时间 TD（STD）组，3）长时间 TD（LTD）组，4）保护（PRP/P）组和 5）治疗（PRP/T）组。通过顺时针扭转右侧睾丸 1080°2.5 小时诱导睾丸缺血。PRP（10μl）在复位前 5 分钟（PRP/P）和复位后 3 小时（PRP/T）内经睾丸内注射。实验结束时，分别于第 2、30、2 和 30 天处死各组 2-5 只大鼠。评估睾丸中一氧化氮、丙二醛、过氧化氢酶、总抗氧化能力、还原型谷胱甘肽、谷胱甘肽-S-转移酶、白细胞介素 1β、肿瘤坏死因子、半胱氨酸天冬氨酸蛋白酶 3 和 B 细胞淋巴瘤 2 的表达。此外，还进行了组织学检查。
PRP 治疗显著减轻了 TD 引起的睾丸退化。特别是通过改善氧化应激状态（NO，P=0.0001）和抗氧化标志物（TAC、GSH、GST，P=0.0001-0.01），降低了 IL-1β、TNF-α和 cas 3 的表达，并增加了 BCL2 倍数变化（P=0.0001）。PRP 的保护作用优于 PRP 的治疗作用，可恢复 TD 后睾丸的组织形态结构。
本研究表明 PRP 对 TD 引起的睾丸细胞损伤具有细胞保护作用，这可能归因于其改善氧化应激和抑制诱导凋亡的能力，提示 PRP 可能作为一种补充疗法应用于不同的睾丸退行性疾病。
