Nano-Biosensors Lab, Department of Biosystems and Agricultural Engineering, Michigan State University, East Lansing, MI 48824, USA.
Global Alliance for Rapid Diagnostics, Michigan State University, East Lansing, MI 48824, USA.
Biosensors (Basel). 2023 Jun 2;13(6):608. doi: 10.3390/bios13060608.
One of the most prevalent causes of foodborne illnesses worldwide is staphylococcal food poisoning. This study aimed to provide a robust method to extract the bacteria from food samples using glycan-coated magnetic nanoparticles (MNPs). Then, a cost-effective multi-probe genomic biosensor was designed to detect the nuc gene of rapidly in different food matrices. This biosensor utilized gold nanoparticles and two DNA oligonucleotide probes combined to produce a plasmonic/colorimetric response to inform users if the sample was positive for . In addition, the specificity and sensitivity of the biosensor were determined. For the specificity trials, the biosensor was compared with the extracted DNA of , serovar Enteritidis (SE), and The sensitivity tests showed that the biosensor could detect as low as 2.5 ng/µL of the target DNA with a linear range of up to 20 ng/µL of DNA. With further research, this simple and cost-effective biosensor can rapidly identify foodborne pathogens from large-volume samples.
全球范围内，食源性疾病的最普遍病因之一是葡萄球菌食物中毒。本研究旨在提供一种强大的方法，使用糖基化磁性纳米颗粒（MNPs）从食物样本中提取细菌。然后，设计了一种具有成本效益的多探针基因组生物传感器，以快速检测不同食物基质中的 nuc 基因。该生物传感器利用金纳米颗粒和两个 DNA 寡核苷酸探针结合，产生等离子体/比色响应，告知用户样品是否对 呈阳性。此外，还确定了生物传感器的特异性和灵敏度。在特异性试验中，将 生物传感器与提取的 、肠炎沙门氏菌（SE）和 的 DNA 进行了比较。灵敏度测试表明，该生物传感器可以检测低至 2.5 ng/µL 的目标 DNA，线性范围高达 20 ng/µL 的 DNA。通过进一步的研究，这种简单且具有成本效益的生物传感器可以从大量样本中快速识别食源性病原体。
