Key Laboratory of Integrated Oncology and Intelligent Medicine of Zhejiang Province, Department of Hepatobiliary and Pancreatic Surgery, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou 310006, China.
Key Laboratory of Surface & Interface Science of Polymer Materials of Zhejiang Province, Department of Chemistry, Zhejiang Sci-Tech University, Hangzhou 310018, China.
Biosensors (Basel). 2023 Jun 16;13(6):661. doi: 10.3390/bios13060661.
Herein we report a highly specific electrochemical aptasenseor for AFB1 determination based on AFB1-controlled diffusion of redox probe (Ru(NH)) through nanochannels of AFB1-specific aptamer functionalized VMSF. A high density of silanol groups on the inner surface confers VMSF with cationic permselectivity, enabling electrostatic preconcentration of Ru(NH) and producing amplified electrochemical signals. Upon the addition of AFB1, the specific interaction between the aptamer and AFB1 occurs and generates steric hindrance effect on the access of Ru(NH), finally resulting in the reduced electrochemical responses and allowing the quantitative determination of AFB1. The proposed electrochemical aptasensor shows excellent detection performance in the range of 3 pg/mL to 3 μg/mL with a low detection limit of 2.3 pg/mL for AFB1 detection. Practical analysis of AFB1 in peanut and corn samples is also accomplished with satisfactory results by our fabricated electrochemical aptasensor.
在此，我们报道了一种基于 AFB1 特异性适体功能化 VMSF 的纳米通道中氧化还原探针（Ru(NH)）扩散控制的 AFB1 高特异性电化学适体传感器。VMSF 内表面高密度的硅醇基团赋予其阳离子选择性，能够静电预浓缩 Ru(NH)并产生放大的电化学信号。加入 AFB1 后，适体与 AFB1 之间发生特异性相互作用，对 Ru(NH)的进入产生空间位阻效应，最终导致电化学响应降低，从而实现对 AFB1 的定量测定。所提出的电化学适体传感器在 3 pg/mL 至 3 μg/mL 的范围内表现出优异的检测性能，对 AFB1 的检测限低至 2.3 pg/mL。我们制备的电化学适体传感器还成功地用于花生和玉米样品中 AFB1 的实际分析，结果令人满意。
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