Department of Naval Nutrition and Food Hygiene, Faculty of Naval Medicine, Naval Medical University, 800 Xiangyin Road, Shanghai, 200433, China; School of Engineering, Huzhou University, Huzhou, 313000, China.
School of Engineering, Huzhou University, Huzhou, 313000, China.
Talanta. 2024 Jun 1;273:125915. doi: 10.1016/j.talanta.2024.125915. Epub 2024 Mar 20.
Aflatoxin (AFs) contamination is one of the serious food safety issues. Aflatoxin B1 (AFB1) is the most common and toxic aflatoxin, which has been classified as a class 1 carcinogen by the International Agency for Research on Cancer (IARC). It is extremely destructive to liver tissue. Developing a convenient and sensitive detection technique is essential. In this paper, we developed a homogeneous dual recognition strategy based electrochemical aptasensor for accurate and sensitive detection of aflatoxin B1 (AFB1) based on the magnetic graphene oxide (MGO) and UiO-66. The MGO was synthesized for the recognition and magnetic separation of AFB1 from complex samples. UiO-66/ferrocenecarboxylic acid (Fc)/aptamer composites were constructed as both recognition and signal probes. The probes would specifically capture AFB1 enriched by MGO, which enables dual recognition in homogeneous solution, thus further improving the accuracy of AFB1 detection. The electrochemical aptasensor for AFB1 had a linear range from 0.005 to 500 ng mL. Additionally, the limit of detection was 1 pg mL. It shows a favorable potential for both sensitive and accurate detection of AFB1 in real samples.
黄曲霉毒素（AFs）污染是严重的食品安全问题之一。黄曲霉毒素 B1（AFB1）是最常见和毒性最强的黄曲霉毒素，被国际癌症研究机构（IARC）列为 1 类致癌物。它对肝组织有极强的破坏性。开发一种方便、灵敏的检测技术至关重要。在本文中，我们基于磁性氧化石墨烯（MGO）和 UiO-66 开发了一种均相双重识别策略的电化学适体传感器，用于准确和灵敏地检测黄曲霉毒素 B1（AFB1）。MGO 用于从复杂样品中识别和分离 AFB1。UiO-66/二茂铁羧酸（Fc）/适体复合物被构建为识别和信号探针。探针会特异性地捕获由 MGO 富集的 AFB1，从而在均相溶液中进行双重识别，从而进一步提高 AFB1 检测的准确性。用于检测 AFB1 的电化学适体传感器的线性范围为 0.005 至 500ng mL。此外，检测限为 1pg mL。它显示出在真实样品中对 AFB1 进行灵敏和准确检测的良好潜力。
