Turpaev T M, Iurchenko O P, Grigor'ev N G
Neirofiziologiia. 1986;18(3):326-32.
The modifying effect of extra- and intracellular administration of serotonin on inward currents generated by the cell at extracellular application of acetylcholine was studied. The study was made on nonidentified isolated neurons of the fresh water mollusc Lymnaea stagnalis using the voltage-clamp and intracellular perfusion methods. In roughly a half of the studied cells serotonin being added intra- and extracellularly produced a decrease in the response to acetylcholine. In the other half of neurons serotonin being added intracellularly increased the acetylcholine response, but applied extracellularly showed the opposite effect: it decreased the magnitude of inward acetylcholine currents. The blocker of serotonin receptors, cyproheptadine, blocks the enhancing effect of serotonin on the intracellular addition of the mediator, but mimics the inhibitory effect of serotonin on acetylcholine response. The data obtained on the neurons of molluscs suggest the existence of intracellular receptors of serotonin one whose possible functions is the regulation of sensitivity of the cell surface cholinoreceptors.
研究了细胞外和细胞内给予血清素对细胞在细胞外应用乙酰胆碱时产生的内向电流的调节作用。该研究使用电压钳和细胞内灌注方法，对淡水软体动物椎实螺未鉴定的分离神经元进行。在大约一半被研究的细胞中，细胞外和细胞内添加血清素会使对乙酰胆碱的反应降低。在另一半神经元中，细胞内添加血清素会增加对乙酰胆碱的反应，但细胞外应用则显示出相反的效果：它会降低内向乙酰胆碱电流的幅度。血清素受体阻滞剂赛庚啶可阻断血清素对细胞内添加介质的增强作用，但模拟血清素对乙酰胆碱反应的抑制作用。在软体动物神经元上获得的数据表明存在血清素的细胞内受体，其可能的功能之一是调节细胞表面胆碱能受体的敏感性。
