Bodmer R, Levitan I B
J Neurobiol. 1984 Nov;15(6):429-40. doi: 10.1002/neu.480150604.
Aplysia neurons grown in primary cell culture (Dagan and Levitan, 1981) were exposed to the putative neurotransmitters acetylcholine and serotonin by local iontophoretic application, and changes in membrane potential or voltage clamp currents were examined. It was found that 47% of the neurons were sensitive to cholinergic agonists, 14% to serotonin, and 9% responded to both. Responses could be recorded upon application of the transmitters to the cell bodies as well as along the regenerated neurites. An identified group of neurons, the neurosecretory bag cells, exhibited similar responses to cholinergic agonists in culture and in situ. Pleural medial neurons exhibited cholinergic responses in culture similar to those previously reported in situ. Thus neurotransmitter receptor/ion channel complexes characteristic for a specific cell type in the intact ganglion are also present on this cell type in culture.
在原代细胞培养中生长的海兔神经元（达甘和莱维坦，1981年）通过局部离子电泳施加假定的神经递质乙酰胆碱和血清素，并检测膜电位或电压钳电流的变化。发现47%的神经元对胆碱能激动剂敏感，14%对血清素敏感，9%对两者都有反应。将递质施加到细胞体以及再生神经突上时均可记录到反应。一组已确定的神经元，即神经分泌囊泡细胞，在培养物中和原位对胆碱能激动剂表现出相似的反应。胸膜内侧神经元在培养物中表现出的胆碱能反应与先前在原位报道的相似。因此，完整神经节中特定细胞类型所特有的神经递质受体/离子通道复合物在培养的该细胞类型中也存在。
