Quijia Christian Rafael, Navegante Geovana, Sábio Rafael Miguel, Valente Valeria, Ocaña Alberto, Alonso-Moreno Carlos, Frem Regina Célia Galvão, Chorilli Marlus
Department of Drugs and Medicines, School of Pharmaceutical Sciences, São Paulo State University (UNESP), Rodovia Araraquara Jau, Km 01-s/n-Campos Ville, Araraquara 14800-903, Brazil.
Department of Clinical Analysis, School of Pharmaceutical Sciences, São Paulo State University (UNESP), Rodovia Araraquara Jau, Km 01-s/n-Campos Ville, Araraquara 14800-903, Brazil.
J Funct Biomater. 2023 Jun 11;14(6):319. doi: 10.3390/jfb14060319.
Piperine (PIP), a compound found in , has shown promise as a potential chemotherapeutic agent for breast cancer. However, its inherent toxicity has limited its application. To overcome this challenge, researchers have developed PIP@MIL-100(Fe), an organic metal-organic framework (MOF) that encapsulates PIP for breast cancer treatment. Nanotechnology offers further treatment options, including the modification of nanostructures with macrophage membranes (MM) to enhance the evasion of the immune system. In this study, the researchers aimed to evaluate the potential of MM-coated MOFs encapsulated with PIP for breast cancer treatment. They successfully synthesized MM@PIP@MIL-100(Fe) through impregnation synthesis. The presence of MM coating on the MOF surface was confirmed through SDS-PAGE analysis, which revealed distinct protein bands. Transmission electron microscopy (TEM) images demonstrated the existence of a PIP@MIL-100(Fe) core with a diameter of around 50 nm, surrounded by an outer lipid bilayer layer measuring approximately 10 nm in thickness. Furthermore, the researchers evaluated the cytotoxicity indices of the nanoparticles against various breast cancer cell lines, including MCF-7, BT-549, SKBR-3, and MDA. The results demonstrated that the MOFs exhibited between 4 and 17 times higher cytotoxicity (IC) in all four cell lines compared to free PIP (IC = 193.67 ± 0.30 µM). These findings suggest that MM@PIP@MIL-100(Fe) holds potential as an effective treatment for breast cancer. The study's outcomes highlight the potential of utilizing MM-coated MOFs encapsulated with PIP as an innovative approach for breast cancer therapy, offering improved cytotoxicity compared to free PIP alone. Further research and development are warranted to explore the clinical translation and optimize the efficacy and safety of this treatment strategy.
胡椒碱（PIP）是一种存在于[具体来源未给出]中的化合物，已显示出作为乳腺癌潜在化疗药物的前景。然而，其固有的毒性限制了它的应用。为了克服这一挑战，研究人员开发了PIP@MIL-100(Fe)，这是一种用于包裹PIP以治疗乳腺癌的有机金属有机框架（MOF）。纳米技术提供了更多的治疗选择，包括用巨噬细胞膜（MM）修饰纳米结构以增强对免疫系统的逃避。在这项研究中，研究人员旨在评估包裹有PIP的MM涂层MOF用于乳腺癌治疗的潜力。他们通过浸渍合成成功地合成了MM@PIP@MIL-100(Fe)。通过SDS-PAGE分析证实了MOF表面存在MM涂层，该分析揭示了明显的蛋白条带。透射电子显微镜（TEM）图像显示存在一个直径约50 nm的PIP@MIL-100(Fe)核心，周围是一层厚度约10 nm的外部脂质双层。此外，研究人员评估了纳米颗粒对各种乳腺癌细胞系的细胞毒性指数，包括MCF-7、BT-549、SKBR-3和MDA。结果表明，与游离PIP（IC = 193.67 ± 0.30 µM）相比，MOF在所有四种细胞系中的细胞毒性（IC）高4至17倍。这些发现表明，MM@PIP@MIL-100(Fe)具有作为乳腺癌有效治疗方法的潜力。该研究的结果突出了利用包裹有PIP的MM涂层MOF作为乳腺癌治疗创新方法的潜力，与单独的游离PIP相比，其细胞毒性有所提高。有必要进行进一步的研究和开发，以探索这种治疗策略的临床转化并优化其疗效和安全性。
