Han Shuncai, Ai Qianqian, Xia Xiaofeng
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Insects. 2023 May 31;14(6):504. doi: 10.3390/insects14060504.
(L.), commonly known as the diamondback moth, is currently a major worldwide pest. Gut bacteria play an important role in the physiology and insecticide resistance of , but little is known about the sources and transmission routes of its gut bacteria. In this study, traditional microbial culture methods were used to analyze the sources and transmission modes of gut bacteria in , which could help develop pest control strategies based on gut bacteria. The main findings are as follows: gut bacterial diversity was significantly higher in -fed radish sprouts than those fed an artificial diet, indicating a potential association between gut bacteria and food bacteria. In addition, sequence analysis revealed the isolation of sp., sp., sp., and sp. from both radish sprouts and . Importantly, sp. was found in all tested samples (radish sprouts, gut of , ovaries, and eggs), suggesting that bacteria acquired from food could be transferred from the gut to the ovaries and eggs. This was confirmed through experiments, which also showed that eggs could carry bacteria and transmit them to the gut, indicating vertical transmission of gut bacteria via eggs. Furthermore, the 3rd instar larvae of with and without gut bacteria were mixed and raised until the 4th instar. Then, we found that all the gut of the 4th instar larvae carried the same bacteria, indicating that the gut bacteria of can be horizontally transmitted through social behavior. This study lays a foundation for further exploration of the sources, transmission, and coevolution of the host of gut bacteria in , and provides new insights into pest control strategies based on the source and transmission of gut bacteria.
小菜蛾（Plutella xylostella (L.)），通常被称为小菜蛾，是目前全球范围内的主要害虫。肠道细菌在小菜蛾的生理和抗药性方面发挥着重要作用，但对其肠道细菌的来源和传播途径知之甚少。在本研究中，采用传统微生物培养方法分析小菜蛾肠道细菌的来源和传播模式，这有助于制定基于肠道细菌的害虫控制策略。主要研究结果如下：以萝卜芽为食的小菜蛾肠道细菌多样性显著高于以人工饲料为食的小菜蛾，表明肠道细菌与食物细菌之间存在潜在关联。此外，序列分析显示从萝卜芽和小菜蛾中均分离出了芽孢杆菌属（Bacillus sp.）、肠杆菌属（Enterobacter sp.）、葡萄球菌属（Staphylococcus sp.）和假单胞菌属（Pseudomonas sp.）。重要的是，在所有测试样本（萝卜芽、小菜蛾肠道、卵巢和卵）中均发现了芽孢杆菌属。这表明从食物中获取的细菌可从肠道转移至卵巢和卵。实验证实了这一点，实验还表明卵可携带细菌并将其传播至肠道，表明肠道细菌可通过卵进行垂直传播。此外，将有无肠道细菌的小菜蛾三龄幼虫混合饲养至四龄。然后，我们发现所有四龄幼虫的肠道都携带相同的细菌，这表明小菜蛾的肠道细菌可通过社会行为进行水平传播。本研究为进一步探索小菜蛾肠道细菌宿主的来源、传播和共同进化奠定了基础，并为基于肠道细菌来源和传播的害虫控制策略提供了新的见解。
