Dr. YSP University of Horticulture and Forestry, 173 212, Solan, HP, India.
ICAR: Central Potato Research Institute, Shimla, HP, India.
Mol Biol Rep. 2022 Aug;49(8):7475-7481. doi: 10.1007/s11033-022-07550-7. Epub 2022 May 18.
Diamondback moth, Plutella xylostella is one of the important pests of cole crops, the larval stages cause damage to leaves from seedling stage to the harvest thus reducing the quality and quantity of the yield. The insect gut posses a large variety of microbial communities among which, the association of bacteria is the most spread and common. Due to variations in various agro-climatic factors, the insect often assumes the status of major pest. These geographical variations in insects influence various biological parameters including insecticide resistance due to diversity of microbes/bacteria. The diverse role of gut bacteria in insect fitness traits has now gained perspectives for biotechnological exploration. The present study was aimed to determine the diversity of larval gut bacteria of diamondback moth collected from five different geographical regions of India. The gut bacteria of this pest were found to be influenced by different geographical regions. A total of 14 larval gut bacterial isolates were obtained. Majority of these bacteria belong to Enterobacteriaceae followed by Yersiniaceae, Morganellaceae and Enterococcaceae. Phylogeny analysis of all the bacterial isolates collected from five different geographical regions of India revealed that all the isolated strains are resolved in well-defined clades with their closest type species.
小菜蛾，也称白菜蛾，是十字花科蔬菜的重要害虫之一，其幼虫阶段会对从幼苗期到收获期的叶片造成损害，从而降低产量的质量和数量。昆虫肠道内存在着大量不同的微生物群落，其中细菌的关联性是最广泛和常见的。由于各种农业气候因素的变化，这种昆虫经常成为主要害虫。昆虫在地理上的这种差异影响了各种生物学参数，包括由于微生物/细菌的多样性而产生的抗药性。肠道细菌在昆虫适应特征方面的多样化作用现在已经成为生物技术探索的重点。本研究旨在确定从印度五个不同地理区域采集的小菜蛾幼虫肠道细菌的多样性。结果表明，这种害虫的肠道细菌受到不同地理区域的影响。共获得了 14 个幼虫肠道细菌分离株。这些细菌主要属于肠杆菌科，其次是耶尔森菌科、摩根菌科和肠球菌科。对从印度五个不同地理区域采集的所有细菌分离株进行的系统发育分析表明，所有分离株都与它们最接近的模式种在明确的进化枝中得到解决。
