Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil.
Universidade Estadual Paulista (Unesp), Instituto de Química, Araraquara, São Paulo, Brazil.
Nat Prod Res. 2024 Jun;38(11):1950-1955. doi: 10.1080/14786419.2023.2225689. Epub 2023 Jun 27.
In the present study, we have evaluated the cytotoxic activity of 282 extracts from 72 native plant species of the Brazilian Atlantic Forest biome. As a result, and leaves extracts showed cytotoxic activity against three tumour cell lines tested (B16F10, SW480 and Jurkat). After bioassay-guided fractionation, the bioactive fractions were submitted to the dereplication study High-performance Liquid Chromatography, connected to High-resolution Mass Spectrometry (HPLC-ESI-QTOF/MS) analysis, combined with a Global Natural Products Social Molecular Networking (GNPS) tool. A combination of bioactivity-guided and dereplication approaches resulted in the putative annotation of 27 clerodane diterpenes and 9 flavonoids as main compounds present in the cytotoxic fractions of . Regarding the active fraction of , 10 megastigmans, 17 spirostane steroids derivatives and 2 lignans were putatively identified. In conclusion, and are potential sources of antitumor compounds.
在本研究中，我们评估了来自巴西大西洋森林生物群落的 72 种本地植物物种的 282 种提取物的细胞毒性活性。结果表明， 和 叶提取物对三种测试的肿瘤细胞系（B16F10、SW480 和 Jurkat）具有细胞毒性活性。经过基于生物活性的分离后，将生物活性部分进行高通量液相色谱-电喷雾飞行时间质谱（HPLC-ESI-QTOF/MS）分析与全球天然产物社会分子网络（GNPS）工具相结合的去重复研究。生物活性指导和去重复方法的结合导致了在 的细胞毒性部分中存在的 27 个 clerodane 二萜和 9 个类黄酮的假定注释。关于 的活性部分，推测鉴定出了 10 个 megastigmans、17 个螺甾烷类固醇衍生物和 2 个木脂素。总之， 和 是抗肿瘤化合物的潜在来源。
